gga-miR-200b-3p Promotes Macrophage Activation and Differentiation via Targeting Monocyte to Macrophage Differentiation-Associated in HD11 Cells.
gga-miR-200b-3p Promotes Macrophage Activation and Differentiation via Targeting Monocyte to Macrophage Differentiation-Associated in HD11 Cells.
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gga-miR-200b-3p 通过将单核细胞靶向 HD11 细胞中的巨噬细胞分化相关来促进巨噬细胞活化和分化
DOI：
10.3389/fimmu.2020.563143
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发表时间：
2020
影响因子：
7.3
通讯作者：
Luo Q
中科院分区：
文献类型：
--
作者：
Lin W;Zhou L;Liu M;Zhang D;Yan Y;Chang YF;Zhang X;Xie Q;Luo Q
MicroRNAs （miRNAs）通过转录后调控基因表达，在多种生物过程中发挥着关键作用。虽然miRNAs参与哺乳动物细胞的增殖和分化，但关于宿主miRNAs对鸟类巨噬细胞活化和分化的影响的报道很少。在这里，我们报道了gga-miR-200b-3p作为正调节因子，在禽类模型中增强巨噬细胞的激活和分化。我们发现，在HD11细胞中，gga-miR-200b-3p的异位表达增加了mhc - ii阳性细胞的数量，促进了促炎细胞因子的表达，并且gga-miR-200b-3p直接靶向单核细胞到巨噬细胞分化相关（MMD）。gga-miR-200b-3p对MMD的抑制增强了HD11细胞的活化和分化，增加了促炎细胞因子的表达。总之，这些发现强调了gga-miR-200b-3p在鸟类巨噬细胞激活和分化中的关键作用。
MicroRNAs (miRNAs) play a critical role in various biological processes through regulation of gene expression post-transcriptionally. Although miRNAs are involved in cell proliferation and differentiation in mammals, few reports regarding the effects of host miRNAs on macrophage activation and differentiation are available in birds. Here, we reported that gga-miR-200b-3p acts as a positive regulator, enhancing macrophage activation and differentiation using an avian model. We found that ectopic expression of gga-miR-200b-3p in HD11 cells enhances the amount of MHC-II-positive cells and promotes the expression of pro-inflammatory cytokines and that gga-miR-200b-3p directly targets monocyte to macrophage differentiation-associated (MMD). The inhibition of MMD by gga-miR-200b-3p enhances the activation and differentiation of HD11 cells and increases the expression of pro-inflammatory cytokines. Collectively, these findings highlight a crucial role of gga-miR-200b-3p in macrophage activation and differentiation in birds.
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影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
影响因子：
64.8
作者：
Johnnidis, Jonathan B.;Harris, Marian H.;Camargo, Fernando D.
通讯作者：
Camargo, Fernando D.
影响因子：
3.5
作者：
Bräuer, AU;Nitsch, R;Savaskan, NE
通讯作者：
Savaskan, NE
影响因子：
2.8
作者：
Liu, Qiang;Zheng, Jin;Han, Hua
通讯作者：
Han, Hua
影响因子：
64.5
作者：
BEUG, H;VONKIRCHBACH, A;GRAF, T
通讯作者：
GRAF, T