Upregulation of microRNA-96-5p is associated with adolescent idiopathic scoliosis and low bone mass phenotype.
Upregulation of microRNA-96-5p is associated with adolescent idiopathic scoliosis and low bone mass phenotype.
复制标题
microRNA-96-5p 的上调与青少年特发性脊柱侧凸和低骨量表型相关
DOI：
10.1038/s41598-022-12938-3
复制
发表时间：
2022-06-11
影响因子：
4.6
通讯作者：
Lee, Wayne Yuk-wai
中科院分区：
文献类型：
--
作者：
Chen, Huanxiong;Yang, Kenneth Guangpu;Zhang, Jiajun;Cheuk, Ka-yee;Nepotchatykh, Evguenia;Wang, Yujia;Hung, Alec Lik-hang;Lam, Tsz-ping;Moreau, Alain;Lee, Wayne Yuk-wai
骨密度测定显示青少年特发性脊柱侧凸（AIS）患者骨量低，其预测曲线进展的预后潜力。最近的研究表明AIS中存在不同的循环mirna，但其诊断潜力及其与低骨量的联系尚未得到充分证明。本研究旨在比较AIS和非脊柱侧凸受试者收集的骨组织中的miRNA谱，并探讨所选择的miRNA候选物是否可以作为AIS的有用诊断生物标志物。微阵列分析发现miR-96-5p是候选基因中上调最多的。在100名AIS女孩和52名健康女孩的血浆样本中测量miR-96-5p水平。我们的研究结果显示，AIS女孩血浆中miR-96-5p水平显著升高，诊断准确性曲线下面积（AUC）为0.671。由血浆miR-96-5p和患者特异性参数（年龄、体重和月经初潮后的年份）组成的模型的AUC改善为0.752。匠心途径分析（Ingenuity Pathway Analysis， IPA）表明骨代谢途径与miR-96-5p之间存在功能联系。综上所述，AIS骨和血浆样本中差异表达的mirna代表了AIS发病机制中疾病生物标志物和参与者的新来源，这需要进一步的验证研究，包括长期随访的男女AIS患者。
Bone densitometry revealed low bone mass in patients with adolescent idiopathic scoliosis (AIS) and its prognostic potential to predict curve progression. Recent studies showed differential circulating miRNAs in AIS but their diagnostic potential and links to low bone mass have not been well-documented. The present study aimed to compare miRNA profiles in bone tissues collected from AIS and non-scoliotic subjects, and to explore if the selected miRNA candidates could be useful diagnostic biomarkers for AIS. Microarray analysis identified miR-96-5p being the most upregulated among the candidates. miR-96-5p level was measured in plasma samples from 100 AIS and 52 healthy girls. Our results showed significantly higher plasma levels of miR-96-5p in AIS girls with an area under the curve (AUC) of 0.671 for diagnostic accuracy. A model that was composed of plasma miR-96-5p and patient-specific parameters (age, body weight and years since menarche) gave rise to an improved AUC of 0.752. Ingenuity Pathway Analysis (IPA) indicated functional links between bone metabolic pathways and miR-96-5p. In conclusion, differentially expressed miRNAs in AIS bone and plasma samples represented a new source of disease biomarkers and players in AIS etiopathogenesis, which required further validation study involving AIS patients of both genders with long-term follow-up.
登录
查看更多内容
影响因子：
4.6
作者：
García-Giménez JL;Rubio-Belmar PA;Peiró-Chova L;Hervás D;González-Rodríguez D;Ibañez-Cabellos JS;Bas-Hermida P;Mena-Mollá S;García-López EM;Pallardó FV;Bas T
通讯作者：
Bas T
影响因子：
6.2
作者：
Kirmani, Salman;Christen, David;Khosla, Sundeep
通讯作者：
Khosla, Sundeep
影响因子：
6.2
作者：
Heilmeier, Ursula;Hackl, Matthias;Link, Thomas M.
通讯作者：
Link, Thomas M.
影响因子：
3
作者：
Cheng, JCY;Guo, X;Sher, AHL
通讯作者：
Sher, AHL
影响因子：
3.1
作者：
Gevrey, M;Dimopoulos, L;Lek, S
通讯作者：
Lek, S