A quantitative description in three dimensions of oxygen uptake by human red blood cells.
A quantitative description in three dimensions of oxygen uptake by human red blood cells.
复制标题
人体红细胞摄氧量的三个维度的定量描述。
DOI：
10.1016/s0006-3495(84)84226-5
复制
发表时间：
1984
影响因子：
3.4
通讯作者：
Olson,JS
中科院分区：
文献类型：
--
作者：
Vandegriff,KD;Olson,JS
从实验和理论两方面考察了与细胞表面相邻的未搅拌的溶剂层对人红细胞摄氧量的影响。将一维平面薄板模型与更复杂的球面和柱面坐标系进行了比较。尽管平面片算法更简单更快，但在考虑未搅拌的溶剂层时，平面片算法是不充分的。圆柱盘模型最接近地代表了人类红细胞的物理几何形状，是进行定量分析所必需的。在我们的停流快速混合实验中，未搅拌的溶剂层的厚度随着残余湍流的衰减而增大。这一现象已经用基于先前发展的流体力学理论的公式进行了量化。初始的10(-4)cm非搅拌层是在混合过程中产生的，并在流动停止时以(T)0.5的函数迅速扩展。该公式与三维圆柱体方案相结合，用于定量描述不同氧浓度、两种不同外部溶剂粘度和两种不同内部血红素浓度下的吸收时间过程。
Oxygen uptake by human erythrocytes has been examined both experimentally and theoretically in terms of the influence of unstirred solvent layers that are adjacent to the cell surface. A one-dimensional plane sheet model has been compared with more complex spherical and cylindrical coordinate schemes. Although simpler and faster, the plane sheet algorithm is an inadequate representation when unstirred solvent layers are considered. The cylindrical disk model most closely represents the physical geometry of human red cells and is required for a quantitative analysis. In our stopped-flow rapid mixing experiments, the thickness of the unstirred solvent layer expands with time as the residual turbulence decays. This phenomenon has been quantified using a formulation based on previously developed hydrodynamic theories. An initial 10(-4) cm unstirred layer is postulated to occur during mixing and expand rapidly with time by a (t)0.5 function when flow stops. This formula, in combination with the three-dimensional cylinder scheme, has been used to describe quantitatively uptake time courses at various oxygen concentrations, two different external solvent viscosities, and two different internal heme concentrations.
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血红蛋白扩散对红细胞吸收和释放氧气的影响。
DOI：
--
发表时间：
1968
期刊：
Respiration Physiology
影响因子：
--
作者：
Waldemar Moll
通讯作者：
Waldemar Moll
影响因子：
3.5
作者：
J. Coin;J. Olson
通讯作者：
J. Olson
人体红细胞吸收氧气的速率。
影响因子：
4.8
作者：
J. Coin;J. Olson
通讯作者：
J. Olson
停流、快速混合测量配体与血红蛋白和红细胞的结合。
影响因子：
--
作者：
Olson,JS
通讯作者：
Olson,JS
影响因子：
3.5
作者：
Weingarden,M;Mizukami,H;Rice,SA
通讯作者：
Rice,SA