Hox D3 expression in normal and impaired wound healing.
Hox D3 expression in normal and impaired wound healing.
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Hox D3 在正常和受损伤口愈合中表达。
DOI：
10.1006/jsre.2001.6174
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Boudreau,N
中科院分区：
文献类型：
--
作者：
Uyeno,LA;Newman-Keagle,JA;Cheung,I;Hunt,TK;Young,DM;Boudreau,N
我们之前已经证明Hox D3和Hox B3可以促进血管生成。由于血管生成对伤口愈合至关重要，我们在愈合受损的正常和遗传性糖尿病成年小鼠受伤后检测了这些基因在血管系统中的表达。方法对野生型和遗传性糖尿病小鼠线性创伤后0、1、4、7、14天的组织进行原位杂交。评估伤口中Hox D3和Hox B3的表达、血管生成和I型胶原的合成。结果shox B3在未损伤组织中、小血管内皮细胞（ECs）中均有表达，而Hox D3在静息内皮细胞中表达较少。损伤后1天，紧靠创面的血管内皮细胞中Hox D3的表达显著上调，且表达可维持至少7天。在糖尿病小鼠中，Hox B3的表达与野生型小鼠相似。相反，在糖尿病小鼠的伤口修复过程中，内皮细胞中Hox D3的表达明显降低和延迟。在培养的微血管内皮细胞中，Hox D3选择性地诱导高水平的I型胶原mRNA表达。糖尿病动物的Hox d3缺陷伤口也显示I型胶原的表达和沉积减少。结论创面愈合受损的糖尿病小鼠血管生成和I型胶原减少可能与Hox D3表达不足有关，恢复Hox D3表达可促进血管生成和创面修复。
BackgroundWe have previously shown that Hox D3 and Hox B3 can promote angiogenesis. As angiogenesis is essential for wound healing, we examined expression of these genes in the vasculature following wounding in normal and genetically diabetic adult mice with impaired healing.MethodsIn situ hybridization was performed on tissues taken 0, 1, 4, 7, and 14 days following administration of linear wounds in wild-type and genetically diabetic mice. Expression of Hox D3 and Hox B3, angiogenesis, and synthesis of type I collagen were assessed in the wound.ResultsHox B3 was expressed in endothelial cells (ECs) of both medium and small vessels in unwounded tissue, whereas little Hox D3 was detected in resting ECs. Hox D3 expression was significantly upregulated by 1 day after wounding in ECs of vessels immediately adjacent to the wound site, and expression was maintained for at least 7 days. In the diabetic mice, expression of Hox B3 was similar to that of wild-type mice. In contrast, expression of Hox D3 in ECs was significantly lower and delayed during wound repair in diabetic mice. In cultured microvascular ECs, Hox D3 selectively induced high levels of collagen I mRNA expression. Hox D3-deficient wounds of diabetic animals also displayed a reduction in expression and deposition of type I collagen.ConclusionsThese results suggest that reduced angiogenesis and type I collagen in diabetic mice with impaired wound healing may be related to deficient Hox D3 expression, and restoring Hox D3 expression may enhance angiogenesis and wound repair.
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--
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