A cellular model for screening of lactobacilli that can enhance tight junctions
A cellular model for screening of lactobacilli that can enhance tight junctions
复制标题
用于筛选可增强紧密连接的乳酸杆菌的细胞模型
DOI：
10.1039/c6ra24148d
复制
发表时间：
2016-11
期刊：
影响因子：
3.9
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Wang, Gang;Zhao, Jianxin;Zhang, Hao;Chen, Wei
肠道屏障紧密连接（TJs）功能障碍是炎症性肠病（IBD）的重要致病因素之一。越来越多的研究表明，益生菌可以替代抗生素等传统药物治疗IBD。然而，很少有有效的细胞模型可以快速筛选有效的益生菌用于TJ调节。益生菌和PT-麦醇溶蛋白的共孵育已经被一些研究者用于研究细菌的TJ调节功能。在本研究中，进行了修改，以克服这种方法的不足，即，不能排除益生菌对TJ恢复的间接作用。在分段孵育模型中，一些乳杆菌菌株被证明在TJ调节中是有效的，这与用动物模型获得的结果相似。主成分分析表明，改进的分段培养模型比共培养模型更能准确反映益生菌在肠道中的调控模式。这项研究提供了一种新的策略，可以提高快速筛选可用于IBD干预的益生菌的效率。
It is widely believed that the dysfunction of tight junctions (TJs) in the gut barrier is one of the most important pathogenic factors in inflammatory bowel disease (IBD). More and more studies show that probiotics can be engaged as a substitute for traditional medicine such as antibiotics to treat IBD. However, there are very few efficient cellular models that can rapidly screen effective probiotics for TJ regulation. Co-incubation of probiotics and PT–gliadin has been used by some researchers to study the TJ regulatory function of bacteria. In this study, a modification was performed to overcome the deficiency of this method, i.e., that the indirect effect of probiotics on TJ restoration cannot be excluded. In a sectional incubation model, some of the Lactobacillus strains proved to be effective in TJ regulation, which is similar to the result obtained with an animal model. Principal component analysis showed that the modified sectional incubation model reflected the regulatory mode of probiotics in the gut more accurately than that by the co-incubation model. This study provides a new strategy that may improve the efficiency of rapid screening of available probiotics for intervention in IBD.
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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DOI：
10.1016/j.bbadis.2011.08.010
发表时间：
2011-12
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Puppa MJ;White JP;Sato S;Cairns M;Baynes JW;Carson JA
通讯作者：
Carson JA
影响因子：
3.5
作者：
Sander, GR;Cummins, AG;Powell, BC
通讯作者：
Powell, BC
DOI：
10.1016/s0140-6736(10)61143-9
发表时间：
2010-07
期刊：
The Lancet
影响因子：
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作者：
Z. Weizman
通讯作者：
Z. Weizman
影响因子：
4.4
作者：
W. Willis;L. Reid
通讯作者：
W. Willis;L. Reid