Differential effects of growth hormone and insulin-like growth factor I on colony formation of epiphyseal chondrocytes in suspension culture in rats of different ages.
Differential effects of growth hormone and insulin-like growth factor I on colony formation of epiphyseal chondrocytes in suspension culture in rats of different ages.
复制标题
生长激素和胰岛素样生长因子I对不同年龄大鼠悬浮培养骨骺软骨细胞集落形成的差异影响。
作者：
A. Lindahl;J. Isgaard;L. Carlsson;O. Isaksson
采用悬浮培养的方法，研究了人生长激素(HGH)和胰岛素样生长因子I(IGF-I)对大鼠骨骺软骨细胞集落形成的影响。从7日龄、20日龄和28日龄正常雄性大鼠的胫骨近端生长板分离软骨细胞，在含10%新生小牛血清和0.5%琼脂糖稳定的悬液中进行培养。培养14天的7、20、28天大鼠软骨细胞克隆形成率分别为13.7+/-1.9、3.2+/-0.6和3.5+/-0.4。根据菌落大小(菌落直径)对菌落进行分类，并根据菌落大小(克隆效率的分布)估计菌落数量。IGF-I(25~200 ng/ml)可使不同年龄组软骨细胞的集落总数增加，尤其是小集落数增加。然而，与对照组相比，IGF-I并没有显著改变克隆效率的分布。HGH在10~80 ng/ml时促进集落形成，而在160 ng/ml时无明显刺激作用。GH使克隆效率的不对称分布与对照组明显不同，原因是大克隆数增加(直径超过80微米)。在不同浓度的GH和IGF-I延长培养时间(28天)后，GH和IGF-I的差异明显。这些结果表明，在悬浮培养中，GH和IGF-I都能诱导骨痂软骨细胞间的克隆形成，尽管GH和IGF-I在克隆效率分布上的作用不同。观察到GH特别促进了大尺寸集落的形成，这表明GH促进了前软骨细胞的分化，也就是前面提到的年轻软骨细胞。与对照组相比，IGF-I刺激导致较小比例的软骨细胞集落的比例更高，这表明IGF-I刺激骨骺软骨细胞在分化的后期阶段。
The effect of human GH (hGH) and insulin-like growth factor I (IGF-I) on the colony formation of rat epiphyseal chondrocytes was studied in suspension culture. Chondrocytes from epiphyseal growth plates of the proximal tibia of 7-, 20-, and 28-day-old normal male rats were enzymatically isolated and cultured in the presence of 10% newborn calf serum in suspension stabilized with 0.5% agarose. The cloning efficiency (number of formed colonies per 1000 seeded cells) was 13.7 +/- 1.9, 3.2 +/- 0.6, and 3.5 +/- 0.4 for chondrocytes isolated from 7-, 20- and 28-day-old rats and cultured for 14 days, respectively. The colonies were classified according to size (colony diameter), and the number of colonies was estimated as a function of colony size (distribution of cloning efficiency). IGF-I (25-200 ng/ml) increased the total colony number among chondrocytes isolated from the three different age groups and particularly increased the number of small colonies. However, IGF-I did not significantly change the distribution of cloning efficiency as compared to the control group. hGH potentiated colony formation at concentrations of 10-80 ng/ml, but no stimulatory effect of hGH was apparent at a concentration of 160 ng/ml. GH caused an assymetric distribution of cloning efficiency that was significantly different from the control group due to an increased number of large colonies (diameter exceeding 80 microns). The differential effects of GH and IGF-I were apparent after an extended period of culture (28 days) at various concentrations of the peptides. These results show that GH as well as IGF-I induced colony formation among epiphyseal chondrocytes in suspension culture, although the effects of GH and IGF-I are different in terms of distribution of cloning efficiency. The observation that GH particularly potentiated the formation of large size colonies suggests that GH promotes the differentiation of prechondrocytes, or young chondrocytes as suggested earlier. The finding that IGF-I stimulation resulted in a higher proportion of small size chondrocyte colonies, compared to the control group, suggests that IGF-I stimulates epiphyseal chondrocytes at a later stage of differentiation.
培养的人成纤维细胞产生免疫反应性生长调节素的激素控制。
DOI：
10.1172/jci110001
发表时间：
1981
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Clemmons,DR;Underwood,LE;VanWyk,JJ
通讯作者：
VanWyk,JJ
影响因子：
4.8
作者：
D. Klapper;M. Svoboda;J. J. Wyk-J.
通讯作者：
D. Klapper;M. Svoboda;J. J. Wyk-J.
DOI：
10.1073/pnas.81.3.935
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DERCOLE, AJ;STILES, AD;UNDERWOOD, LE
通讯作者：
UNDERWOOD, LE