The promoter region of 46-kDa CNPase is sufficient for its expression in corpus callosum.
The promoter region of 46-kDa CNPase is sufficient for its expression in corpus callosum.
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DOI：
10.1016/j.ymgmr.2018.03.003
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发表时间：
2018-06
影响因子：
1.9
通讯作者：
Yamauchi J
中科院分区：
文献类型：
--
作者：
Miyamoto Y;Torii T;Tanoue A;Yamamoto M;Yamauchi J
髓鞘由中枢神经系统（CNS）中的少突胶质细胞或外周神经系统（PNS）中的许旺细胞形成。它由许多生物有机成分组成，如脂质，蛋白质和氨基酸，以及核苷酸[1，2]。越来越多的证据表明，以遗传性髓鞘生成不足性脑白质营养不良（HLD）为代表的髓鞘疾病与代谢失败有关[3，4]。环核苷酸磷酸二酯酶（CNE）是中枢神经系统中主要的髓鞘组分蛋白之一。CNE 2不仅作为细胞内磷酸二酯酶参与核苷酸代谢，而且还将肌动蛋白细胞骨架连接到髓鞘膜的细胞内侧[1]。cnpase基因编码48-和46-kDa的两种同种型，其由不同的启动子调节[5]。尽管在髓鞘膜内稳态的重要作用，分离的1-羟色胺酶上游单位的mRNA编码的较小的异构体是否真的有助于蛋白质的表达的问题仍然是没有答案的。相比之下，较长的同种型使用分离的1-脱氢酶单元上游的特异性启动子[5]。我们已经成功地获得了一个含有小鼠cnpase基因和cre的分离的1-β-淀粉酶单位的转基因小鼠品系[6，7]（图1A和1B）。S1和S2）。将小鼠与ROSA 26-β-半乳糖苷酶（ROSA 26-BGAL，JAX品系No.003474）小鼠杂交，鉴定Cre重组酶阳性细胞。因此，我们免疫染色新生转基因小鼠脑组织切片垂直的胼胝体，其中含有高密度髓鞘的有髓轴突。BGAL染色
Myelin is formed by oligodendrocytes in the central nervous system (CNS) or by Schwann cells in the peripheral nervous system (PNS). It is composed of many bioorganic components such as lipids, proteins and amino acids, as well as nucleotides [1, 2]. Growing evidence indicates that myelin diseases represented by genetic hypomyelinating leukodystrophies (HLDs) are related to the failure of the metabolism [3, 4]. 2’, 3’-Cyclic nucleotide phosphodiesterase (CNPase) is one of the major myelin component proteins in the CNS. CNPase participates not only in nucleotide metabolism as intracellular phosphodiesterase but also in linking actin cytoskeletons to the intracellular side of myelin membranes [1]. The cnpase gene encodes two isoforms of 48-and 46-kDa, which are regulated by different promoters [5]. Despite the important role of CNPase in myelin membrane homeostasis, the question of whether the isolated 1-kilobase upstream unit from mRNA encoding the smaller isoform actually contributes to protein expression remains to be unanswered. In contrast, the longer isoform uses the specific promoter upstream of the isolated 1-kilobase unit [5]. We have succeeded in getting one line of transgenic mice harboring the isolated 1-kilobase unit of the mouse cnpase gene and cre [6, 7](Figs. S1 and S2). The mice were crossbred with ROSA26-β-galactosidase (ROSA26-BGAL, JAX's strain No. 003474) mice, identifying Cre recombinase-positive cells. We thus immunostained neonatal transgenic mouse brain tissues sliced vertically for myelinated axons in corpus callosum, which contains high-dense myelin sheaths. BGAL staining
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影响因子：
64.8
作者：
Fuenfschilling, Ursula;Supplie, Lotti M.;Mahad, Don;Boretius, Susann;Saab, Aiman S.;Edgar, Julia;Brinkmann, Bastian G.;Kassmann, Celia M.;Tzvetanova, Iva D.;Moebius, Wiebke;Diaz, Francisca;Meijer, Dies;Suter, Ueli;Hamprecht, Bernd;Sereda, Michael W.;Moraes, Carlos T.;Frahm, Jens;Goebbels, Sandra;Nave, Klaus-Armin
通讯作者：
Nave, Klaus-Armin
影响因子：
82.9
作者：
Saher, Gesine;Rudolphi, Fabian;Nave, Klaus-Armin
通讯作者：
Nave, Klaus-Armin
影响因子：
16.2
作者：
SCHERER, SS;BRAUN, PE;KAMHOLZ, J
通讯作者：
KAMHOLZ, J
影响因子：
16.6
作者：
Miyamoto Y;Torii T;Tanoue A;Yamauchi J
通讯作者：
Yamauchi J
MBP和CNP的拮抗功能在CNS髓鞘中建立胞质通道。
影响因子：
8.8
作者：
Snaidero N;Velte C;Myllykoski M;Raasakka A;Ignatev A;Werner HB;Erwig MS;Möbius W;Kursula P;Nave KA;Simons M
通讯作者：
Simons M