Adding bovine seminal plasma prior to freezing improves post-thaw bull sperm kinematics but decreases mitochondrial activity
Adding bovine seminal plasma prior to freezing improves post-thaw bull sperm kinematics but decreases mitochondrial activity
复制标题
在冷冻前添加牛精浆可改善解冻后公牛精子的运动学，但会降低线粒体活性
DOI：
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发表时间：
2018
影响因子：
2.4
通讯作者：
J. Morrell
中科院分区：
文献类型：
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作者：
T. Nongbua;E. Al;A. Edman;A. Johannisson;J. Morrell
摘要 精子质量相当的公牛之间的生育力差异可能是由于精浆 (SP) 的差异造成的。本研究的目的是研究将来自已知生育能力的公牛的牛 SP 添加到不含 SP 的精子样本中的效果。通过单层离心去除 SP 后，在冷冻前用来自高或低生育力公牛的 SP 处理重悬的精子颗粒，浓度为 0%（对照）、1% 或 5%。解冻后评估精子质量。使用 Proc MIXED、SAS® 分析数据。牛 SP 分别为 1% 或 5% SP1 和 SP5 时，平均路径速度、曲线速度和横向头部位移幅度降低，而摆动和线性度增加。此外，与 SP1 和对照相比，SP5 处理组中具有高线粒体膜电位 (MMP) 的精子比例最低。 SP比例不影响精子质量的其他参数。因此，添加5%牛SP对一些精子速度参数产生有利影响，但对MMP产生不利影响。高生育力公牛和低生育力公牛的 SP 效果没有差异。缩写：AI：人工授精； BCF：节拍交叉频率； CASA：计算机辅助精子分析； IVF：体外受精； MMP：线粒体膜电位； SLC：单层离心； SP：精浆
ABSTRACT Variations in fertility between bulls with comparable sperm quality could be due to differences in their seminal plasma (SP). The aim of this study was to investigate the effect of adding bovine SP from bulls of known fertility to SP-free sperm samples. After removal of SP by Single Layer Centrifugation, resuspended sperm pellets were treated with SP from high or low fertility bulls at 0% (control), 1%, or 5% before freezing. Sperm quality was evaluated after thawing. Data were analyzed using Proc MIXED, SAS®. Bovine SP at 1% or 5% SP1 and SP5, respectively, decreased average path velocity, curvilinear velocity, and amplitude of lateral head displacement whereas wobble and linearity were increased. In addition, the proportion of spermatozoa with high mitochondrial membrane potential (MMP) was lowest for treatment with SP5 compared to SP1 and control. The proportion of SP did not affect other parameters of sperm quality. Thus, adding 5% bovine SP produced a favorable effect on some sperm velocity parameters but had an unfavorable effect on MMP. There were no differences in effect between SP from high and low fertility bulls. Abbreviations: AI: artificial insemination; BCF: beat cross frequency; CASA: computer-assisted sperm analysis; IVF: in vitro fertilization; MMP: mitochondrial membrane potential; SLC: single layer centrifugation; SP: seminal plasma
影响因子：
1.7
作者：
Suarez, SS;Ho, HC
通讯作者：
Ho, HC