Recording central nervous system responses of freely-swimming marine and freshwater fishes with a customizable, implantable AC differential amplifier

Recording central nervous system responses of freely-swimming marine and freshwater fishes with a customizable, implantable AC differential amplifier
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使用可定制的植入式交流差分放大器记录自由游动的海洋和淡水鱼类的中枢神经系统反应

DOI:
10.1016/j.jneumeth.2023.109850
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发表时间:
2023
影响因子:
3
通讯作者:
Liao, James C.
Liao, James C.
中科院分区:
医学4区
文献类型:
--
作者:
Gibbs, Brendan J.;Strother, James A.;Liao, James C.

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背景鱼类已经适应了不同的环境,但自然水生行为背后的神经机制尚不清楚。新方法我们开发了一种小型、可定制的交流差分放大器和外科手术程序,用于记录海洋和淡水鱼类中枢神经系统中的多个细胞外信号。结果我们的微创放大器使鱼类能够定向流动,并对水动力和视觉刺激做出反应。我们记录了这些行为过程中小脑和视顶盖的活动。与现有方法相比,我们的系统非常低成本,流体动力学简化,能够获得高增益,以便记录复杂流体环境中自由活动的快速鱼类。结论我们的拴系方法允许在实验室记录各种成鱼的神经活动,但也可以进行修改,以便在野外记录数据。
BackgroundFish have adapted to a diversity of environments but the neural mechanisms underlying natural aquatic behaviors are not well known.New methodWe have developed a small, customizable AC differential amplifier and surgical procedures for recording multi-unit extracellular signals in the CNS of marine and freshwater fishes.ResultsOur minimally invasive amplifier allowed fish to orient to flow and respond to hydrodynamic and visual stimuli. We recorded activity in the cerebellum and optic tectum during these behaviors.Comparison with existing methodsOur system is very low-cost, hydrodynamically streamlined, and capable of high-gain in order to allow for recordings from freely behaving, fast fishes in complex fluid environments.ConclusionsOur tethered approach allows access to record neural activity in a diversity of adult fishes in the lab, but can also be modified for data logging in the field.
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