Overexpression of PIK3R1 promotes hepatocellular carcinoma progression.
Overexpression of PIK3R1 promotes hepatocellular carcinoma progression.
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PIK3R1过度表达促进肝细胞癌进展
DOI：
10.1186/s40659-018-0202-7
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发表时间：
2018-11-29
影响因子：
6.7
通讯作者：
Jiang T
中科院分区：
文献类型：
--
作者：
Ai X;Xiang L;Huang Z;Zhou S;Zhang S;Zhang T;Jiang T
磷酸肌醇-3-激酶，调节亚基1（PIK 3R 1）可以调节癌细胞增殖，对癌细胞增殖至关重要;然而，其在肝细胞癌（HCC）中的作用在很大程度上仍然未知。在这里，我们研究了PIK 3R 1在HCC中的作用，并研究了潜在的分子机制。通过免疫组织化学和qRT-PCR评估PIK 3R 1在一系列HCC组织中的表达。采用qRT-PCR和western blot检测PIK 3R 1在人肝癌细胞系中的mRNA和蛋白表达，并以MHCC 97 H和HCCLM 3细胞为模型，研究PIK 3R 1在肝癌进展中的作用。通过MTT法、克隆形成实验、创伤愈合实验和流式细胞术检测PIK 3R 1基因敲除对肝癌细胞增殖、迁移和凋亡的影响。Western blot检测PI 3 K/AKT/mTOR信号通路的表达变化。我们的研究发现，PIK 3R 1在肝癌组织中的表达高于癌旁正常组织。敲低PIK 3R 1可抑制肝癌细胞的增殖、迁移和促进凋亡。此外，我们证明敲低PIK 3R 1下调MHCC 97 H和HCCLM 3细胞中p-PI 3 K、p-AKT和p-mTOR的表达。PIK 3R 1为肝癌的治疗提供了潜在的新靶点。
Phosphoinositide-3-kinase, regulatory subunit 1 (PIK3R1) could regulate cancer cell proliferation important for cancer cell proliferation; however, its role in Hepatocellular carcinoma (HCC) remains largely unknown. Here, we investigated the role of PIK3R1 in HCC and examined the underlying molecular mechanisms. The expression of PIK3R1 was evaluated by immunohistochemistry and qRT-PCR in a series of HCC tissues. The mRNA and protein expression of PIK3R1 was used by qRT-PCR and western blot assays in a series of human HCC cell lines, and then we choose MHCC97H and HCCLM3 cells as a model to investigate the effect of PIK3R1 on HCC progression. The effects of PIK3R1 knowdown on cell proliferation, migration, apoptosis of HCC were assessed by the MTT assay, clonogenic assays, wound healing assay and flow cytometry in vitro. Western blot assay was performed to assess the expression changes of PI3K/AKT/mTOR signaling pathway. Our results found that PIK3R1 was highly expressed in HCC tissues compared with adjacent normal tissues. Knockdown of PIK3R1 inhibited the proliferation, migration and promoted apoptosis of HCC cell lines. In addition, we proved that knockdown of PIK3R1 downregulated p-PI3K, p-AKT, and p-mTOR expressions in MHCC97H and HCCLM3 cells. In conclusion, PIK3R1 providing potential novel targets for the treatment of HCC.
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影响因子：
2.9
作者：
Zhuo ZJ;Xiao MJ;Lin HR;Luo J;Wang T
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影响因子：
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作者：
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影响因子：
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作者：
Chikara S;Lindsey K;Borowicz P;Christofidou-Solomidou M;Reindl KM
通讯作者：
Reindl KM
DOI：
10.1007/978-3-319-42118-6_3
发表时间：
2016-01-01
期刊：
METABOLISM IN CANCER
影响因子：
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作者：
Lien, Evan C.;Lyssiotis, Costas A.;Cantley, Lewis C.
通讯作者：
Cantley, Lewis C.
影响因子：
30.8
作者：
Fruman, DA;Mauvais-Jarvis, F;Cantley, LC
通讯作者：
Cantley, LC