Trypsin- and chymotrypsin-induced chemiluminescence by isolated rat glomeruli.
Trypsin- and chymotrypsin-induced chemiluminescence by isolated rat glomeruli.
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胰蛋白酶和胰凝乳蛋白酶诱导的离体大鼠肾小球化学发光。
DOI：
10.1152/ajpcell.1987.252.6.c611
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Shah,SV
中科院分区：
文献类型：
--
作者：
Basci,A;Shah,SV
We examined whether the generation of reactive oxygen metabolites (as quantified by measuring luminol-amplified chemiluminescence) by isolated rat glomeruli could be triggered enzymatically. No response was observed with thrombin (1 or 10 U/ml), collagenase (100, 200, or 400 U/ml), or plasmin (0.1 or 1 U/ml). In contrast, chymotrypsin and trypsin caused a dose-dependent (10-200 micrograms/ml) increase in chemiluminescence from glomeruli. The peak response with chymotrypsin (100 micrograms/ml) and trypsin (50 micrograms/ml) was as follows: resting, 16 +/- 2 X 10(3) cpm/mg protein, n = 17; chymotrypsin, 233 +/- 58 X 10(3) cpm/mg protein, n = 17; and trypsin, 221 +/- 38 X 10(3) cpm/mg protein, n = 10. Tubules had only a minor response. Soybean trypsin inhibitor and aprotinin caused marked inhibition, indicating the dependency of the chemiluminescence response on the protease enzyme activity. The chemiluminescence response was by glomeruli rather than by "contaminating" leukocytes, since a similar marked response (n = 6) was observed in glomeruli isolated from cyclophosphamide-treated leukopenic (leukocyte less than 1,000/mm3) rats. Superoxide dismutase, a scavenger of superoxide, and free-radical scavengers benzoate and tryptophan inhibited the glomerular chemiluminescence response to trypsin and chymotrypsin. Neutral proteases from infiltrating leukocytes and/or renal tissue have been shown to be released in glomerular diseases; our results, which show the generation of chemiluminescence in response to neutral proteases, suggest a potential mechanism for the production of reactive oxygen metabolites in glomerular diseases.
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通过蛋白水解酶暴露人血小板上的纤维蛋白原受体。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Niewiarowski,S;Budzynski,AZ;Morinelli,TA;Brudzynski,TM;Stewart,GJ
通讯作者：
Stewart,GJ
凝血酶、胰蛋白酶和离子载体 A 23187 刺激内皮细胞产生前列环素。
影响因子：
15.9
作者：
B. Weksler;C. W. Ley;E. Jaffe
通讯作者：
E. Jaffe
胰凝乳蛋白酶对血小板释放反应和聚集的影响
DOI：
10.3181/00379727-165-40943
发表时间：
1980
影响因子：
--
作者：
R. Grant;M. Zucker
通讯作者：
M. Zucker
有证据表明蛋白酶参与人类多形核白细胞和单核细胞产生超氧化物。
DOI：
10.1172/jci109662
发表时间：
1980
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
S. Kitagawa;F. Takaku;S. Sakamoto
通讯作者：
S. Sakamoto
[39] 吞噬细胞产生化学发光（CL）
影响因子：
--
作者：
M. Trush;M. Wilson;K. Dyke
通讯作者：
K. Dyke