A hinge migration mechanism unlocks the evolution of green-to-red photoconversion in GFP-like proteins.
A hinge migration mechanism unlocks the evolution of green-to-red photoconversion in GFP-like proteins.
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DOI：
10.1016/j.str.2014.11.011
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发表时间：
2015-01-06
期刊：
影响因子：
5.7
通讯作者：
Wachter, Rebekka M.
中科院分区：
文献类型：
--
作者：
Kim, Hanseong;Zou, Taisong;Modi, Chintan;Doerner, Katerina;Grunkemeyer, Timothy J.;Chen, Liqing;Fromme, Raimund;Matz, Mikhail V.;Ozkan, S. Banu;Wachter, Rebekka M.
In proteins, functional divergence involves mutations that modify structure and dynamics. Here, we provide experimental evidence for an evolutionary mechanism driven solely by long-range dynamic motions without significant backbone adjustments, catalytic group rearrangements, or changes in subunit assembly. Crystallographic structures were determined for several reconstructed ancestral proteins belonging to a GFP class frequently employed in superresolution microscopy. Their chain flexibility was analyzed using molecular dynamics and perturbation response scanning. The green-tored photoconvertible phenotype appears to have arisen from a common green ancestor by migration of a knob-like anchoring region away from the active site diagonally across the beta-barrel fold. The allosterically coupled mutational sites provide active site conformational mobility via epistasis. We propose that light-induced chromophore twisting is enhanced in a reverse-protonated subpopulation, activating internal acid-base chemistry and backbone cleavage to enlarge the chromophore. Dynamics-driven hinge migration may represent a more general platform for the evolution of novel enzyme activities.
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DOI：
10.1016/j.str.2009.06.008
发表时间：
2009-08-12
期刊：
Structure (London, England : 1993)
影响因子：
--
作者：
del Sol A;Tsai CJ;Ma B;Nussinov R
通讯作者：
Nussinov R
影响因子：
3.7
作者：
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通讯作者：
Matz MV
DOI：
10.1107/s090744499900846x
发表时间：
1999-10-01
期刊：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
影响因子：
--
作者：
Leslie, AGW
通讯作者：
Leslie, AGW
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
2.9
作者：
Elsliger, MA;Wachter, RM;Remington, SJ
通讯作者：
Remington, SJ