Nitrate regulates floral induction in Arabidopsis, acting independently of light, gibberellin and autonomous pathways.
Nitrate regulates floral induction in Arabidopsis, acting independently of light, gibberellin and autonomous pathways.
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DOI：
10.1007/s00425-010-1316-5
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发表时间：
2011-03
期刊：
影响因子：
4.3
通讯作者：
Osuna D
中科院分区：
文献类型：
--
作者：
Castro Marín I;Loef I;Bartetzko L;Searle I;Coupland G;Stitt M;Osuna D
从营养生长到生殖的转变是植物发育的一个重要事件。为了最大限度地提高繁殖成功率，它的时间是由环境线索（如光周期，温度和营养可用性）与内部遗传程序之间的复杂相互作用决定的。虽然光周期-和温度-和赤霉酸-信号通路已进行了广泛的分析，很少有人知道营养物质如何调节成花诱导。这部分是因为营养供应对营养生长也有很大的影响，使得很难区分对开花的主要和次要影响。建立了一个使用谷氨酰胺补充剂的生长系统，以允许硝酸盐变化而不会对氨基酸和蛋白质水平或生长速率产生大的影响。在硝态氮限制条件下，C24、Col-0和莱尔在中性（12/12）或短日照（8/16）条件下开花更快。在光周期、温度、赤霉素和自主开花途径受损的晚花突变体中，在fca co-2 ga 1 -3三重突变体和ft-7 soc 1 -1双突变体中，低硝酸盐仍然加速开花，这表明硝酸盐在其他已知的成花诱导途径的下游起作用。其他几种非生物胁迫没有触发fca co-2 ga 1 -3的开花，这表明硝酸盐不是通过一般的胁迫途径起作用的。低硝酸盐并没有进一步加速开花长日（16/8）或在35 S：：CO线，并覆盖晚开花表型的35 S：：FLC线。我们的结论是，低硝酸盐诱导开花通过一种新的信号通路，作用下游，但相互作用，已知的成花诱导途径。本文的在线版本（doi：10.1007/s 00425 -010-1316-5）包含补充材料，可供授权用户使用。
The transition from vegetative growth to reproduction is a major developmental event in plants. To maximise reproductive success, its timing is determined by complex interactions between environmental cues like the photoperiod, temperature and nutrient availability and internal genetic programs. While the photoperiod- and temperature- and gibberellic acid-signalling pathways have been subjected to extensive analysis, little is known about how nutrients regulate floral induction. This is partly because nutrient supply also has large effects on vegetative growth, making it difficult to distinguish primary and secondary influences on flowering. A growth system using glutamine supplementation was established to allow nitrate to be varied without a large effect on amino acid and protein levels, or the rate of growth. Under nitrate-limiting conditions, flowering was more rapid in neutral (12/12) or short (8/16) day conditions in C24, Col-0 and Laer. Low nitrate still accelerated flowering in late-flowering mutants impaired in the photoperiod, temperature, gibberellic acid and autonomous flowering pathways, in the fca co-2 ga1-3 triple mutant and in the ft-7 soc1-1 double mutant, showing that nitrate acts downstream of other known floral induction pathways. Several other abiotic stresses did not trigger flowering in fca co-2 ga1-3, suggesting that nitrate is not acting via general stress pathways. Low nitrate did not further accelerate flowering in long days (16/8) or in 35S::CO lines, and did override the late-flowering phenotype of 35S::FLC lines. We conclude that low nitrate induces flowering via a novel signalling pathway that acts downstream of, but interacts with, the known floral induction pathways. The online version of this article (doi:10.1007/s00425-010-1316-5) contains supplementary material, which is available to authorized users.
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