Nucleoid occlusion prevents cell division during replication fork arrest in Bacillus subtilis.
Nucleoid occlusion prevents cell division during replication fork arrest in Bacillus subtilis.
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DOI：
10.1111/j.1365-2958.2010.07369.x
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发表时间：
2010-11
影响因子：
3.6
通讯作者：
Rudner DZ
中科院分区：
文献类型：
--
作者：
Bernard R;Marquis KA;Rudner DZ
细菌如何应对染色体复制压力一直是传统上研究使用温度敏感的突变体和化学抑制剂。这些方法不可避免地阻止所有复制并导致转录反应的诱导和细胞分裂的抑制。在这里，我们使用阻遏蛋白结合操纵基因阵列，以产生一个单一的停滞复制叉。这些复制障碍阻碍了一个臂上的复制体进展，使另一个臂的复制和重新启动不受影响。值得注意的是，尽管在路障期间RecA-GFP细丝的稳健产生和对细胞分裂的强烈阻断，但基因表达模式没有显著改变。与这些研究结果一致，分裂抑制不是由SOS诱导的调节剂YneA介导的，也不是由RecA独立的抑制ftsL。为了支持这一观点，即类核闭塞防止不适当的细胞分裂过程中叉逮捕，未成熟的FtsZ环形成的DNA质量附近，但很少在其上。此外，轻微的改变染色体压实导致细胞分裂，截断的DNA。引人注目的是，类核蛋白Noc在分裂抑制中没有明显的作用。我们的数据表明，Noc独立的类核闭塞防止不适当的细胞分裂复制叉逮捕期间。他们进一步表明，枯草芽孢杆菌通常管理复制应激，而不是诱导应激反应。
How bacteria respond to chromosome replication stress has been traditionally studied using temperature-sensitive mutants and chemical inhibitors. These methods inevitably arrest all replication and lead to induction of transcriptional responses and inhibition of cell division. Here, we used repressor proteins bound to operator arrays to generate a single stalled replication fork. These replication roadblocks impeded replisome progression on one arm, leaving replication of the other arm and re-initiation unaffected. Remarkably, despite robust generation of RecA-GFP filaments and a strong block to cell division during the roadblock, patterns of gene expression were not significantly altered. Consistent with these findings, division inhibition was not mediated by the SOS-induced regulator YneA nor by RecA-independent repression of ftsL. In support of the idea that nucleoid occlusion prevents inappropriate cell division during fork arrest, immature FtsZ-rings formed adjacent to the DNA mass but rarely on top of it. Furthermore, mild alterations in chromosome compaction resulted in cell division that guillotined the DNA. Strikingly, the nucleoid occlusion protein Noc had no discernable role in division inhibition. Our data indicate that Noc-independent nucleoid occlusion prevents inappropriate cell division during replication fork arrest. They further suggest that Bacillus subtilis normally manages replication stress rather than inducing a stress-response.
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影响因子：
3.2
作者：
Goranov, Alexi I.;Kuester-Schoeck, Elke;Grossman, Alan D.
通讯作者：
Grossman, Alan D.
影响因子：
3.2
作者：
Daniel, Richard A.;Noirot-Gros, Marie-Francoise;Errington, Jeff
通讯作者：
Errington, Jeff
DOI：
10.1016/0006-291x(73)90521-4
发表时间：
1973-01-01
影响因子：
3.1
作者：
COZZARELLI, NR;LOW, RL
通讯作者：
LOW, RL
DOI：
10.1073/pnas.70.1.103
发表时间：
1973-01-01
影响因子：
11.1
作者：
GASS, KB;LOW, RL;COZZARELLI, NR
通讯作者：
COZZARELLI, NR
影响因子：
64.5
作者：
Gruber, Stephan;Errington, Jeff
通讯作者：
Errington, Jeff