High-quality evergreen azalea genome reveals tandem duplication-facilitated low-altitude adaptability and floral scent evolution.
High-quality evergreen azalea genome reveals tandem duplication-facilitated low-altitude adaptability and floral scent evolution.
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高质量的常绿杜鹃花基因组揭示了串联复制促进的低海拔适应性和花香进化
DOI：
10.1111/pbi.13680
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发表时间：
2021-12
影响因子：
13.8
通讯作者：
Xia Y
中科院分区：
文献类型：
--
作者：
Wang X;Gao Y;Wu X;Wen X;Li D;Zhou H;Li Z;Liu B;Wei J;Chen F;Chen F;Zhang C;Zhang L;Xia Y
杜鹃花属于杜鹃花属，杜鹃花是开花植物中最大的属之一，以其1000多个木本物种的多样性和美丽而闻名。杜鹃花有两个不同的类群：高海拔的种类最多，低海拔的种类也很少;然而，高海拔的种类很难驯化用于城市园林绿化，它们的进化和适应性也鲜为人知。卵形杜鹃在低海拔地区具有广泛的适应性，但具有高海拔物种的万年青特性，并且具有大多数品种所缺乏的花香。在这里，我们报告了卵形红球藻染色体水平的基因组组装，其总长度为549 Mb，骨架N50为41 Mb，包含41 264个预测基因。通过与两种高海拔杜鹃的基因组微进化分析，发现卵形杜鹃的扩展基因在防御反应中显著富集，这可能是其适应低海拔的原因。与失去花香的物种相比，卵形杜鹃基因组含有更多的萜烯合酶基因（TPS）。TPS的亚家族B成员参与倍半萜和单萜的合成，并在植物群气味生物合成和防御反应中发挥重要作用。串联重复是驱动防御反应基因扩展的主要力量，以广泛适应低海拔环境。卵叶杜鹃基因组为杜鹃属植物的低海拔适应和花香的获得或损失提供了新的见解，这对高山植物驯化和花香育种具有重要价值。
Azalea belongs to Rhododendron, which is one of the largest genera of flowering plants and is well known for the diversity and beauty in its more than 1000 woody species. Rhododendron contains two distinct groups: the most high‐altitude and a few low‐altitude species; however, the former group is difficult to be domesticated for urban landscaping, and their evolution and adaptation are little known. Rhododendron ovatum has broad adaptation in low‐altitude regions but possesses evergreen characteristics like high‐altitude species, and it has floral fragrance that is deficient in most cultivars. Here we report the chromosome‐level genome assembly of R. ovatum, which has a total length of 549 Mb with scaffold N50 of 41 Mb and contains 41 264 predicted genes. Genomic micro‐evolutionary analysis of R. ovatum in comparison with two high‐altitude Rhododendron species indicated that the expansion genes in R. ovatum were significantly enriched in defence responses, which may account for its adaptability in low altitudes. The R. ovatum genome contains much more terpene synthase genes (TPSs) compared with the species that lost floral fragrance. The subfamily b members of TPS are involved in the synthesis of sesquiterpenes as well as monoterpenes and play a major role in flora scent biosynthesis and defence responses. Tandem duplication is the primary force driving expansion of defence‐responsive genes for extensive adaptability to the low‐altitude environments. The R. ovatum genome provides insights into low‐altitude adaptation and gain or loss of floral fragrance for Rhododendron species, which are valuable for alpine plant domestication and floral scent breeding.
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DOI：
10.1007/978-3-319-90698-0_11
发表时间：
2018-01-01
期刊：
ORNAMENTAL CROPS
影响因子：
--
作者：
De Riek, Jan;De Keyser, Ellen;Kobayashi, Nobuo
通讯作者：
Kobayashi, Nobuo
影响因子：
5.8
作者：
Goll, Johannes;Rusch, Douglas B.;Yooseph, Shibu
通讯作者：
Yooseph, Shibu
影响因子：
48
作者：
Chin, Chen-Shan;Peluso, Paul;Schatz, Michael C.
通讯作者：
Schatz, Michael C.
影响因子：
18
作者：
Chaw, Shu-Miaw;Liu, Yu-Ching;Tsai, Isheng J.
通讯作者：
Tsai, Isheng J.
Soapnuke：MAPREDUCE加速支持的软件，用于集成质量控制和预处理高通量测序数据。
影响因子：
9.2
作者：
Chen Y;Chen Y;Shi C;Huang Z;Zhang Y;Li S;Li Y;Ye J;Yu C;Li Z;Zhang X;Wang J;Yang H;Fang L;Chen Q
通讯作者：
Chen Q