Antibodies to β2-Glycoprotein I Inhibit Phospholipid Dependent Coagulation Reactions
Antibodies to β2-Glycoprotein I Inhibit Phospholipid Dependent Coagulation Reactions
复制标题
β2-糖蛋白 I 抗体抑制磷脂依赖性凝血反应
作者：
J. Brandt
狼疮抗凝剂是在体外抑制磷脂依赖性凝血反应的抗体。这些抗体具有临床意义，因为它们与以微血管血栓形成为特征的多种临床表现相关。虽然这些抗体最初被认为是针对带负电荷的磷脂，但最近的研究表明，它们可能是针对磷脂-蛋白质复合物。研究了抗β2-糖蛋白I（β2-GP I，载脂蛋白H）抗体对磷脂依赖性凝血反应的影响。抗β2-GP Ⅰ的多克隆抗体和单克隆抗体均能抑制凝血酶的生成，并呈剂量依赖性。凝血酶形成的抑制是由于与β2-GP I的特异性相互作用。没有证据表明抑制是由于与凝血酶原酶复合物中涉及的其他蛋白质的交叉反应性。这些发现证明，针对β2-GP I的抗体可以具有与狼疮抗凝活性类似的抗凝活性，并且与最近在狼疮抗凝患者样本中观察到的此类活性一致。
Summary Lupus anticoagulants are antibodies that inhibit phospholipid dependent coagulation reactions in vitro. These antibodies are of clinical interest because of their association with a variety of clinical manifestations characterized by microvascular thrombosis. Although these antibodies were originally thought to be directed at negatively charged phospholipid, recent studies have suggested that they may be directed at phospholipid-protein complexes. The effect of antibodies directed against β2-glycoprotein I (β2-GP I, apolipoprotein H) on phospholipid-dependent coagulation reactions has been studied. Polyclonal and monoclonal antibodies to β2-GP I were found to inhibit thrombin generation in a dose dependent manner. Inhibition of thrombin formation was due to specific interaction with β2-GP I. There was no evidence that inhibition was due to crossreactivity with other proteins involved in the prothrombinase complex. These findings document that antibodies directed against β2-GP I can have anticoagulant activity analogous to lupus anticoagulant activity and are consistent with the recent observation of such activity in lupus anticoagulant patient samples.
影响因子：
15.9
作者：
GHARAVI, AE;SAMMARITANO, LR;ELKON, KB
通讯作者：
ELKON, KB
影响因子：
20.3
作者：
Rebecca A. Fleck;S. Rapaport;Vijaya Mohan Rao
通讯作者：
Rebecca A. Fleck;S. Rapaport;Vijaya Mohan Rao