Effects of hydrostatic pressure on growth and luminescence of a moderately-piezophilic luminous bacteria Photobacterium phosphoreum ANT-2200.
Effects of hydrostatic pressure on growth and luminescence of a moderately-piezophilic luminous bacteria Photobacterium phosphoreum ANT-2200.
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DOI：
10.1371/journal.pone.0066580
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Tamburini C
中科院分区：
文献类型：
--
作者：
Martini S;Al Ali B;Garel M;Nerini D;Grossi V;Pacton M;Casalot L;Cuny P;Tamburini C
细菌生物发光现象在深海中很常见，取决于环境条件。发光杆菌ANT-2200是从西北地中海靠近ANTARES中微子望远镜的2200米深处（现场温度为13°C）分离出来的。在受控的实验室条件下，利用专门开发的高压生物发光系统，研究了静水压力对其生长和发光的影响。在不同的温度（从4 ° C至37°C）和压力（从0.1 MPa至40 MPa）下测定菌株的生长速率和最大群体密度，使用逻辑模型来定义这两个生长参数。实际上，仅使用生长速率，不能确定最佳温度和压力。然而，当增长率和最大种群密度共同考虑时，计算交叉系数。通过这种方式，发现P. phosphoreum ANT-2200的最佳生长条件为30°C和IOMPa，将该菌株定义为嗜温和中度嗜压。此外，不饱和与饱和的细胞脂肪酸的比率被发现在22 MPa下更高，与先前描述的嗜压菌株一致。P. phosphoreum ANT-2200似乎也通过形成细胞聚集体来响应高压。其最大种群密度是0.1 MPa时的1.2倍，增长率相似。在22 MPa下生长的菌株ANT-2200产生3倍以上的生物发光。拟议的方法尽可能接近模拟现场条件，可以帮助研究深海细菌生物发光，并验证关于其在深海碳循环中的作用的假设。
Bacterial bioluminescence is commonly found in the deep sea and depends on environmental conditions. Photobacterium phosphoreum ANT-2200 has been isolated from the NW Mediterranean Sea at 2200-m depth (in situ temperature of 13°C) close to the ANTARES neutrino telescope. The effects of hydrostatic pressure on its growth and luminescence have been investigated under controlled laboratory conditions, using a specifically developed high-pressure bioluminescence system. The growth rate and the maximum population density of the strain were determined at different temperatures (from 4 to 37°C) and pressures (from 0.1 to 40 MPa), using the logistic model to define these two growth parameters. Indeed, using the growth rate only, no optimal temperature and pressure could be determined. However, when both growth rate and maximum population density were jointly taken into account, a cross coefficient was calculated. By this way, the optimum growth conditions for P. phosphoreum ANT-2200 were found to be 30°C and, 10 MPa defining this strain as mesophile and moderately piezophile. Moreover, the ratio of unsaturated vs. saturated cellular fatty acids was found higher at 22 MPa, in agreement with previously described piezophile strains. P. phosphoreum ANT-2200 also appeared to respond to high pressure by forming cell aggregates. Its maximum population density was 1.2 times higher, with a similar growth rate, than at 0.1 MPa. Strain ANT-2200 grown at 22 MPa produced 3 times more bioluminescence. The proposed approach, mimicking, as close as possible, the in situ conditions, could help studying deep-sea bacterial bioluminescence and validating hypotheses concerning its role into the carbon cycle in the deep ocean.
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影响因子：
56.9
作者：
Azam, F
通讯作者：
Azam, F
影响因子：
5.1
作者：
Grossi, Vincent;Yakimov, Michail M.;Tamburini, Christian
通讯作者：
Tamburini, Christian
影响因子：
2.5
作者：
Al Ali, Badr;Garel, Marc;Tamburini, Christian
通讯作者：
Tamburini, Christian
影响因子：
64.8
作者：
ANDREWS, CC;KARL, DM;FOWLER, SW
通讯作者：
FOWLER, SW
影响因子：
56.9
作者：
DELONG, EF;YAYANOS, AA
通讯作者：
YAYANOS, AA