A microfluidic cell co-culture platform with a liquid fluorocarbon separator.
A microfluidic cell co-culture platform with a liquid fluorocarbon separator.
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DOI：
10.1007/s10544-014-9834-8
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发表时间：
2014-04
影响因子：
2.8
通讯作者：
Li, Deyu
中科院分区：
文献类型：
--
作者：
Brewer, Bryson M.;Shi, Mingjian;Edd, Jon F.;Webb, Donna J.;Li, Deyu
已经开发了使用液体氟碳油屏障将细胞分离到不同培养室中的微流体细胞共培养平台。表征表明，油屏障可以有效持续多天，并且在油屏障失效之前，细胞培养室中的油屏障和水性培养基之间的最大压差可以大至103.43kPa。用两种不同的荧光蛋白分别转染两组原代海马神经元，随后观察神经元之间的突触接触，已经证明了生物学应用。此外，通过测试每个装置阻断用于对培养室中的细胞进行染色的低分子量CellTracker染料的能力，显示出由油屏障提供的流体密封的质量大于替代的固体-PDMS阀屏障设计的流体密封的质量。
A microfluidic cell co-culture platform that uses a liquid fluorocarbon oil barrier to separate cells into different culture chambers has been developed. Characterization indicates that the oil barrier could be effective for multiple days, and a maximum pressure difference between the oil barrier and aqueous media in the cell culture chamber could be as large as ∼3.43 kPa before the oil barrier fails. Biological applications have been demonstrated with the separate transfection of two groups of primary hippocampal neurons with two different fluorescent proteins and subsequent observation of synaptic contacts between the neurons. In addition, the quality of the fluidic seal provided by the oil barrier is shown to be greater than that of an alternative solid-PDMS valve barrier design by testing the ability of each device to block low molecular weight CellTracker dyes used to stain cells in the culture chambers.
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影响因子：
11.2
作者：
Brantley-Sieders DM;Dunaway CM;Rao M;Short S;Hwang Y;Gao Y;Li D;Jiang A;Shyr Y;Wu JY;Chen J
通讯作者：
Chen J
影响因子：
7.4
作者：
Hibara, A;Nonaka, M;Kitamori, T
通讯作者：
Kitamori, T
影响因子：
6.1
作者：
Mazutis, Linas;Griffiths, Andrew D.
通讯作者：
Griffiths, Andrew D.
影响因子：
7.4
作者：
Kane, Bartholomew J.;Zinner, Michael J.;Toner, Mehmet
通讯作者：
Toner, Mehmet
影响因子：
6.1
作者：
Fordyce, P. M.;Diaz-Botia, C. A.;Gomez-Sjoberg, R.
通讯作者：
Gomez-Sjoberg, R.