Redesigning Enzymes for Biocatalysis: Exploiting Structural Understanding for Improved Selectivity.
Redesigning Enzymes for Biocatalysis: Exploiting Structural Understanding for Improved Selectivity.
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DOI：
10.3389/fmolb.2022.908285
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发表时间：
2022
影响因子：
5
通讯作者：
中科院分区：
文献类型：
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作者：
新酶的发现，以及使化学过程更加可持续的努力，导致工业界对利用生物催化过程生产高价值和手性前体化学品的兴趣日益浓厚。蛋白质工程方法和计算机工具的巨大进步促进了生物催化过程酶的发现和生产方面的重大进展。然而，我们对酶结构和功能之间关系的了解存在很大差距。这表明需要改进计算方法来建模机制和理解结构动力学。在这里，我们探索合理修饰酶以改变其催化化学方面的努力。我们重点介绍了一些酶的例子，其中酶的功能和结构之间已经建立了联系，从而能够对酶结构进行合理的改变，从而产生可预测的化学结果。我们着眼于该领域未来的发展方向以及实现该领域的技术。
The discovery of new enzymes, alongside the push to make chemical processes more sustainable, has resulted in increased industrial interest in the use of biocatalytic processes to produce high-value and chiral precursor chemicals. Huge strides in protein engineering methodology and in silico tools have facilitated significant progress in the discovery and production of enzymes for biocatalytic processes. However, there are significant gaps in our knowledge of the relationship between enzyme structure and function. This has demonstrated the need for improved computational methods to model mechanisms and understand structure dynamics. Here, we explore efforts to rationally modify enzymes toward changing aspects of their catalyzed chemistry. We highlight examples of enzymes where links between enzyme function and structure have been made, thus enabling rational changes to the enzyme structure to give predictable chemical outcomes. We look at future directions the field could take and the technologies that will enable it.
单一氨基酸的取代可逆转抗生素五烯内酯生物合成中拜尔-维利格单加氧酶 PntE 的区域特异性。
影响因子：
2.9
作者：
Chen, Ke;Wu, Shiwen;Zhu, Lu;Zhang, Chengde;Xiang, Wensheng;Deng, Zixin;Ikeda, Haruo;Cane, David E.;Zhu, Dongqing
通讯作者：
Zhu, Dongqing