A multifaceted study of stigma/style cysteine-rich adhesin (SCA)-like Arabidopsis lipid transfer proteins (LTPs) suggests diversified roles for these LTPs in plant growth and reproduction.
A multifaceted study of stigma/style cysteine-rich adhesin (SCA)-like Arabidopsis lipid transfer proteins (LTPs) suggests diversified roles for these LTPs in plant growth and reproduction.
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DOI：
10.1093/jxb/erq228
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发表时间：
2010-10
影响因子：
6.9
通讯作者：
Lord EM
中科院分区：
文献类型：
--
作者：
Chae K;Gonong BJ;Kim SC;Kieslich CA;Morikis D;Balasubramanian S;Lord EM
百合柱头/花柱富含半胱氨酸的粘附素（SCA）是一种分泌到细胞外基质中的植物脂质转运蛋白（LTP），在受精过程中起着引导花粉管生长的作用。拟南芥LTP 5是一种SCA样分子，其功能获得性突变体（ltp 5 -1）最近被证明在有性生殖中表现出缺陷。在目前的研究中，据报道ltp 5 -1植物具有矮化的初生芽，延迟的下胚轴伸长，各种异常的组织融合，并显示多分枝。这些突变体在营养生长中的表型是隐性的。在ltp 5 -1/+植株中未发现异常。在植物LTP的系统发育分析中，SCA样拟南芥LTP与常规植物LTP分类。同源建模为基础的静电相似性指数（ESI）聚类被用来显示SCA样LTP的静电电位的空间分布的多样性，提示它们在细胞外基质空间的相互作用中的各种作用。β-葡萄糖醛酸酶（GUS）分析表明，SCA类拟南芥LTP基因在不同组织中存在差异。LTP 4特异性地存在于保卫细胞中，LTP 6特异性地存在于毛状体以及其它组织中。LTP 1在柱头中含量丰富，而LTP 3和LTP 6在胚珠中含量丰富。20%聚乙二醇（PEG）和300 mM NaCl处理，分别上调LTP 2和LTP 4基因水平在整个幼苗。在黑暗生长条件下，下胚轴中LTP 5表达上调。在干旱胁迫条件下，LTP 6在子叶的顶端特异性表达。这些结果表明，SCA样拟南芥LTP是多功能的，在植物生长和繁殖中具有多样化的作用。
Lily stigma/style cysteine-rich adhesin (SCA), a plant lipid transfer protein (LTP) which is secreted into the extracellular matrix, functions in pollen tube guidance in fertilization. A gain-of-function mutant (ltp5-1) for Arabidopsis LTP5, an SCA-like molecule, was recently shown to display defects in sexual reproduction. In the current study, it is reported that ltp5-1 plants have dwarfed primary shoots, delayed hypocotyl elongation, various abnormal tissue fusions, and display multibranching. These mutant phenotypes in vegetative growth are recessive. No abnormality was found in ltp5-1/+ plants. In a phylogenetic analysis of plant LTPs, SCA-like Arabidopsis LTPs were classified with conventional plant LTPs. Homology modelling-based electrostatic similarity index (ESI) clustering was used to show diversity in spatial distributions of electrostatic potentials of SCA-like LTPs, suggestive of their various roles in interaction in the extracellular matrix space. β-Glucuronidase (GUS) analysis showed that SCA-like Arabidopsis LTP genes are diversely present in various tissues. LTP4 was found specifically in the guard cells and LTP6 in trichomes as well as in other tissues. LTP1 levels were specifically abundant in the stigma, and both LTP3 and LTP6 in the ovules. LTP2 and LTP4 gene levels were up-regulated in whole seedlings with 20% polyethylene glycol (PEG) and 300 mM NaCl treatments, respectively. LTP5 was up-regulated in the hypocotyl with 3 d dark growth conditions. LTP6 was specifically expressed in the tip of the cotyledon under drought stress conditions. The results suggest that SCA-like Arabidopsis LTPs are multifunctional, with diversified roles in plant growth and reproduction.
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