Induction of Id-1 by FGF-2 involves activity of EGR-1 and sensitizes neuroblastoma cells to cell death.
Induction of Id-1 by FGF-2 involves activity of EGR-1 and sensitizes neuroblastoma cells to cell death.
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DOI：
10.1002/jcp.22505
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发表时间：
2011-07
影响因子：
5.6
通讯作者：
Peruzzi, Francesca
中科院分区：
文献类型：
--
作者：
Passiatore, Giovanni;Gentilella, Antonio;Rom, Slava;Pacifici, Marco;Bergonzini, Valeria;Peruzzi, Francesca
分化抑制因子-1（Inhibitor of differentiation-1，Id-1）是螺旋-环-螺旋（helix-loop-helix，HLH）蛋白家族的一员，通过与碱性HLH转录因子的抑制性结合来调节基因转录。与该家族的其他成员类似，Id-1参与细胞分化的抑制和细胞生长的激活。Id-1的双重功能，即抑制分化和刺激细胞增殖，可能是相互依赖的，因为细胞分化通常与退出细胞周期相关联。成纤维细胞生长因子-2（FGF-2）在中枢神经系统（CNS）发育过程中的不同生物学过程中发挥着多种作用。此外，FGF-2已被描述为诱导神经母细胞瘤SK-N-MC细胞中的“神经元样”分化并触发细胞凋亡。虽然几种促有丝分裂因子对Id-1蛋白的调节是公认的，但对FGF-2在Id-1调节中的作用知之甚少。使用人神经母细胞瘤细胞系SK-N-MC，我们发现，这些细胞与FGF-2的治疗导致早期诱导Id-1 mRNA和蛋白。诱导发生在FGF-2处理后1小时内，并由ERK 1/2途径介导，ERK 1/2途径反过来刺激早期生长反应-1（Egr-1）转录因子的表达。我们还证明了Egr-1与Id-1启动子在体外和细胞培养中的直接相互作用。最后，Id-1表达的抑制导致FGF-2处理的细胞的G2/M积累和延迟的细胞死亡。
Inhibitor of differentiation-1 (Id-1) is a member of helix–loop–helix (HLH) family of proteins that regulate gene transcription through their inhibitory binding to basic-HLH transcription factors. Similarly to other members of this family, Id-1 is involved in the repression of cell differentiation and activation of cell growth. The dual function of Id-1, inhibition of differentiation, and stimulation of cell proliferation, might be interdependent, as cell differentiation is generally coupled with the exit from the cell cycle. Fibroblast growth factor-2 (FGF-2) has been reported to play multiple roles in different biological processes during development of the central nervous system (CNS). In addition, FGF-2 has been described to induce “neuronal-like” differentiation and trigger apoptosis in neuroblastoma SK-N-MC cells. Although regulation of Id-1 protein by several mitogenic factors is well-established, little is known about the role of FGF-2 in the regulation of Id-1. Using human neuroblastoma cell line, SK-N-MC, we found that treatment of these cells with FGF-2 resulted in early induction of both Id-1 mRNA and protein. The induction occurs within 1 h from FGF-2 treatment and is mediated by ERK1/2 pathway, which in turn stimulates expression of the early growth response-1 (Egr-1) transcription factor. We also demonstrate direct interaction of Egr-1 with Id-1 promoter in vitro and in cell culture. Finally, inhibition of Id-1 expression results in G2/M accumulation of FGF-2-treated cells and delayed cell death.
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影响因子：
5.7
作者：
Baird, Andrew
通讯作者：
Baird, Andrew
影响因子：
8
作者：
Gentilella, A.;Passiatore, G.;Khalili, K.
通讯作者：
Khalili, K.
DOI：
10.1016/0955-2235(92)90026-e
发表时间：
1992-01-01
期刊：
Progress in Growth Factor Research
影响因子：
--
作者：
Ledoux, Dominique;Gannoun-Zaki, Leila;Barritault, Denis
通讯作者：
Barritault, Denis
影响因子：
64.5
作者：
BENEZRA, R;DAVIS, RL;WEINTRAUB, H
通讯作者：
WEINTRAUB, H
影响因子：
4.8
作者：
Higgins, S.;Wong, S. H. X.;Russo, V. C.
通讯作者：
Russo, V. C.