Differentiation between Oppositely Oriented Microtubules Controls Polarized Neuronal Transport.
Differentiation between Oppositely Oriented Microtubules Controls Polarized Neuronal Transport.
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DOI：
10.1016/j.neuron.2017.11.018
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发表时间：
2017-12-20
期刊：
影响因子：
16.2
通讯作者：
Kapitein LC
中科院分区：
文献类型：
--
作者：
Tas RP;Chazeau A;Cloin BMC;Lambers MLA;Hoogenraad CC;Kapitein LC
微管对神经元的极化运输是必不可少的，但它们的组织如何将马达蛋白引导到轴突或树突尚不清楚。因为不同的发动机识别不同的微管属性，我们使用光学纳米显微镜来检查微管方向、稳定性和修饰之间的关系。马达对超分辨微管及其极性的纳米跟踪表明，在树枝晶中，稳定的和乙酰化的微管大多是负端向外的，而动态的和酪氨酸化的微管的取向是相反的。此外，具有相似取向和修饰的微管形成偏向传输的束。重要的是，由于正端定向的kinesin-1选择性地与乙酰化的微管相互作用，这个组织引导这个马达走出树突，进入轴突。相比之下，Kinesin-3更喜欢酪氨酸化的微管，可以进入轴突和树突。这种将不同的微管亚群分离成相反方向的束构成了神经元微管细胞骨架的关键结构原理，使不同的马达蛋白能够进行极化分选。基于电机的纳米显微镜可以直接观察神经元中微管(MT)的极性，MT组织成极化的束在树突中局部偏向运输，相反方向的束稳定性不同，组成树突的MTS偏向Kinesin-1向胞体运输，确保轴突选择性Tas等。使用光学纳米显微镜显示，相反取向的树突状微管在稳定性和组成上存在差异，并招募不同的运动蛋白。这就解释了为什么有些马达蛋白可以进入树突，而另一些只能进入轴突。
Microtubules are essential for polarized transport in neurons, but how their organization guides motor proteins to axons or dendrites is unclear. Because different motors recognize distinct microtubule properties, we used optical nanoscopy to examine the relationship between microtubule orientations, stability, and modifications. Nanometric tracking of motors to super-resolve microtubules and determine their polarity revealed that in dendrites, stable and acetylated microtubules are mostly oriented minus-end out, while dynamic and tyrosinated microtubules are oriented oppositely. In addition, microtubules with similar orientations and modifications form bundles that bias transport. Importantly, because the plus-end-directed Kinesin-1 selectively interacts with acetylated microtubules, this organization guides this motor out of dendrites and into axons. In contrast, Kinesin-3 prefers tyrosinated microtubules and can enter both axons and dendrites. This separation of distinct microtubule subsets into oppositely oriented bundles constitutes a key architectural principle of the neuronal microtubule cytoskeleton that enables polarized sorting by different motor proteins. Motor-based nanoscopy enables direct observation of microtubule (MT) polarity In neurons, MTs organize into polarized bundles that locally bias transport In dendrites, bundles of opposite orientation differ in stability and composition Dendritic MTs bias Kinesin-1 transport toward the soma, ensuring axon selectivity Tas et al. use optical nanoscopy to show that dendritic microtubules of opposite orientation differ in stability and composition and recruit different motor proteins. This explains why some motor proteins can move into dendrites while others can only enter axons.
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影响因子：
16.2
作者：
Jacobson, C;Schnapp, B;Banker, GA
通讯作者：
Banker, GA
影响因子：
9.2
作者：
Kapitein, Lukas C.;Schlager, Max A.;Hoogenraad, Casper C.
通讯作者：
Hoogenraad, Casper C.
微管蛋白代码：分子成分、读出机制和功能。
DOI：
10.1083/jcb.201406055
发表时间：
2014-08-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Janke C
通讯作者：
Janke C
影响因子：
3.3
作者：
Hammond JW;Huang CF;Kaech S;Jacobson C;Banker G;Verhey KJ
通讯作者：
Verhey KJ
影响因子：
9.2
作者：
Kapitein, Lukas C.;van Bergeijk, Petra;Hoogenraad, Casper C.
通讯作者：
Hoogenraad, Casper C.