The genetic architecture of genome-wide recombination rate variation in allopolyploid wheat revealed by nested association mapping.
The genetic architecture of genome-wide recombination rate variation in allopolyploid wheat revealed by nested association mapping.
复制标题
DOI：
10.1111/tpj.14009
复制
发表时间：
2018-09
期刊：
影响因子：
--
通讯作者：
Akhunov ED
中科院分区：
文献类型：
--
作者：
Jordan KW;Wang S;He F;Chao S;Lun Y;Paux E;Sourdille P;Sherman J;Akhunova A;Blake NK;Pumphrey MO;Glover K;Dubcovsky J;Talbert L;Akhunov ED
变异通过对遗传信息的重组施加限制来影响种群中等位基因的命运。了解这些限制的遗传基础是至关重要的操纵重组过程，以提高遗传作图的分辨率，并减少连锁阻力和有害的遗传负荷在育种中的负面影响。利用基于序列的基因分型小麦巢式关联作图（NAM）群体的2，100个重组自交系通过杂交29个不同的线，我们定位QTL影响102 000个交叉（CO）的分布和频率。全基因组重组率变异主要由具有小影响的罕见等位基因定义，共同解释高达48.6%的变异。大多数QTL是加性的，主要表现为反式作用。影响近端CO的QTL也起加性作用，而不增加远端CO的频率。我们发现，与重组率高的区域相比，重组率降低的区域携带更多的单核苷酸多态性（SNP），可能具有有害影响。因此，我们的研究提供了深入了解小麦重组率变异的遗传基础及其对有害SNP在基因组中分布的影响。鉴定的反式作用加性QTL可用于操纵大多倍体小麦基因组中的CO频率和分布，从而有可能提高基因重组的效率并减少染色体的低重组近着丝粒区域中的有害遗传负荷。这项研究提供了异源多倍体小麦重组率变异的遗传基础及其对有害SNP在基因组中分布的影响的见解。所鉴定的反式作用加性重组率QTL可用于操纵大型多倍体小麦基因组中的交换频率和分布，从而有可能提高基因重组的效率并减少染色体的低重组近着丝粒区域中的有害遗传负荷。
Recombination affects the fate of alleles in populations by imposing constraints on the reshuffling of genetic information. Understanding the genetic basis of these constraints is critical for manipulating the recombination process to improve the resolution of genetic mapping, and reducing the negative effects of linkage drag and deleterious genetic load in breeding. Using sequence‐based genotyping of a wheat nested association mapping (NAM) population of 2,100 recombinant inbred lines created by crossing 29 diverse lines, we mapped QTL affecting the distribution and frequency of 102 000 crossovers (CO). Genome‐wide recombination rate variation was mostly defined by rare alleles with small effects together explaining up to 48.6% of variation. Most QTL were additive and showed predominantly trans‐acting effects. The QTL affecting the proximal COs also acted additively without increasing the frequency of distal COs. We showed that the regions with decreased recombination carry more single nucleotide polymorphisms (SNPs) with possible deleterious effects than the regions with a high recombination rate. Therefore, our study offers insights into the genetic basis of recombination rate variation in wheat and its effect on the distribution of deleterious SNPs across the genome. The identified trans‐acting additive QTL can be utilized to manipulate CO frequency and distribution in the large polyploid wheat genome opening the possibility to improve the efficiency of gene pyramiding and reducing the deleterious genetic load in the low‐recombining pericentromeric regions of chromosomes. This study offers insights into the genetic basis of recombination rate variation in allopolyploid wheat and its effect on the distribution of deleterious SNPs across the genome. The identified trans‐acting additive recombination rate QTL can be utilized to manipulate crossover frequency and distribution in the large polyploid wheat genome opening the possibility to improve the efficiency of gene pyramiding and reducing the deleterious genetic load in the low recombining pericentromeric regions of chromosomes.
登录
查看更多内容
影响因子：
4.4
作者：
Akhunov ED;Akhunova AR;Anderson OD;Anderson JA;Blake N;Clegg MT;Coleman-Derr D;Conley EJ;Crossman CC;Deal KR;Dubcovsky J;Gill BS;Gu YQ;Hadam J;Heo H;Huo N;Lazo GR;Luo MC;Ma YQ;Matthews DE;McGuire PE;Morrell PL;Qualset CO;Renfro J;Tabanao D;Talbert LE;Tian C;Toleno DM;Warburton ML;You FM;Zhang W;Dvorak J
通讯作者：
Dvorak J
影响因子：
12.3
作者：
Bauer E;Falque M;Walter H;Bauland C;Camisan C;Campo L;Meyer N;Ranc N;Rincent R;Schipprack W;Altmann T;Flament P;Melchinger AE;Menz M;Moreno-González J;Ouzunova M;Revilla P;Charcosset A;Martin OC;Schön CC
通讯作者：
Schön CC
影响因子：
3.3
作者：
Hartfield, Matthew;Glemin, Sylvain
通讯作者：
Glemin, Sylvain
影响因子：
56.9
作者：
Buckler, Edward S.;Holland, James B.;McMullen, Michael D.
通讯作者：
McMullen, Michael D.
影响因子：
14.9
作者：
De Baets G;Van Durme J;Reumers J;Maurer-Stroh S;Vanhee P;Dopazo J;Schymkowitz J;Rousseau F
通讯作者：
Rousseau F