Investigation of tension wood formation and 2,6-dichlorbenzonitrile application in short rotation coppice willow composition and enzymatic saccharification.
Investigation of tension wood formation and 2,6-dichlorbenzonitrile application in short rotation coppice willow composition and enzymatic saccharification.
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DOI：
10.1186/1754-6834-4-13
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发表时间：
2011-05-24
影响因子：
6.3
通讯作者：
Murphy RJ
中科院分区：
文献类型：
--
作者：
Brereton NJ;Pitre FE;Ray MJ;Karp A;Murphy RJ
在英国和其他地方，短轮丛柳是一种潜在的木质纤维素原料；然而，专门用于生物乙醇生产的优化柳树的研究直到最近才开始发展。我们以柳（Salix viminalis） ×柳（Salix schwerinii）品种‘Olof’为原料，进行了为期三个月的盆栽试验，目的是改变茎内细胞壁的组成和结构，以促进生物乙醇的生产。用张力木材诱导和/或对次生细胞壁特异的纤维素合成抑制剂2,6-二氯苯腈对树木进行26或43天的处理。反应木（张力木和对向木）是从经过43天张力木诱导处理的材料中分离出来的。测定葡聚糖含量、木质素含量和酶解葡萄糖含量。所有测量的参数都改变了，但茎总生物量产量没有损失，这表明酶解糖化产率可以通过改变细胞壁结构和改变葡聚糖或木质素的绝对含量来提高。最终的葡萄糖产率可以通过诱导张力木材而提高，而不会对生物量产率产生不利影响。增加细胞壁降解酶对葡聚糖的可及性有助于减少木质纤维素生物乙醇生产过程对能源和环境的影响。
Short rotation coppice willow is a potential lignocellulosic feedstock in the United Kingdom and elsewhere; however, research on optimising willow specifically for bioethanol production has started developing only recently. We have used the feedstock Salix viminalis × Salix schwerinii cultivar 'Olof' in a three-month pot experiment with the aim of modifying cell wall composition and structure within the stem to the benefit of bioethanol production. Trees were treated for 26 or 43 days with tension wood induction and/or with an application of the cellulose synthesis inhibitor 2,6-dichlorobenzonitrile that is specific to secondary cell walls. Reaction wood (tension and opposite wood) was isolated from material that had received the 43-day tension wood induction treatment. Glucan content, lignin content and enzymatically released glucose were assayed. All measured parameters were altered without loss of total stem biomass yield, indicating that enzymatic saccharification yield can be enhanced by both alterations to cell wall structure and alterations to absolute contents of either glucan or lignin. Final glucose yields can be improved by the induction of tension wood without a detrimental impact on biomass yield. The increase in glucan accessibility to cell wall degrading enzymes could help contribute to reducing the energy and environmental impacts of the lignocellulosic bioethanol production process.
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