Nitrogen Starvation Impacts the Photosynthetic Performance of Porphyridium cruentum as Revealed by Chlorophyll a Fluorescence.
Nitrogen Starvation Impacts the Photosynthetic Performance of Porphyridium cruentum as Revealed by Chlorophyll a Fluorescence.
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叶绿素 a 荧光揭示氮饥饿影响紫球藻的光合作用性能
DOI：
10.1038/s41598-017-08428-6
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发表时间：
2017-08-17
影响因子：
4.6
通讯作者：
Zhang YZ
中科院分区：
文献类型：
--
作者：
Zhao LS;Li K;Wang QM;Song XY;Su HN;Xie BB;Zhang XY;Huang F;Chen XL;Zhou BC;Zhang YZ
氮是植物和藻类生存所需的最重要的营养物质之一，藻类的光合作用能力与氮的丰度有关。红藻是藻类进化中独特的光合真核生物，其类囊体膜上含有藻胆体(Pbs)。利用多色脉冲幅度调制(PAM)荧光测定仪，研究了氮素缺乏对紫球藻体内叶绿素a荧光动力学的影响。氮素饥饿使PSII的光化学效率和反应中心(RCS)活性下降，并出现光抑制现象。缺氮严重影响了PSII供体侧的水分解系统，导致放氧复合体(OEC)失活，降低了光能转化效率。在氮饥饿的细胞中，更多的能量被用于光化学反应，热耗散减少，如QP和QN所示。氮饥饿细胞耐受和抵抗高光子通量密度的能力减弱。结果表明，氮素缺乏严重影响了紫云英的光合作用。
Nitrogen is one of the most important nutrients needed for plants and algae to survive, and the photosynthetic ability of algae is related to nitrogen abundance. Red algae are unique photosynthetic eukaryotic organisms in the evolution of algae, as they contain phycobilisomes (PBSs) on their thylakoid membranes. In this report, the in vivo chlorophyll (Chl) a fluorescence kinetics of nitrogen-starved Porphyridium cruentum were analyzed to determine the effects of nitrogen deficiency on photosynthetic performance using a multi-color pulse amplitude modulation (PAM) chlorophyll fluorometer. Due to nitrogen starvation, the photochemical efficiency of PSII and the activity of PSII reaction centers (RCs) decreased, and photoinhibition of PSII occurred. The water-splitting system on the donor side of PSII was seriously impacted by nitrogen deficiency, leading to the inactivation of the oxygen-evolving complex (OEC) and decreased light energy conversion efficiency. In nitrogen-starved cells, a higher proportion of energy was used for photochemical reactions, and thermal dissipation was reduced, as shown by qP and qN. The ability of nitrogen-starved cells to tolerate and resist high photon flux densities was weakened. Our results showed that the photosynthetic performance of P. cruentum was severely impacted by nitrogen deficiency.
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影响因子：
5.2
作者：
Ciompi, S;Gentili, E;Soldatini, GF
通讯作者：
Soldatini, GF
影响因子：
3.1
作者：
EILERS, PHC;PEETERS, JCH
通讯作者：
PEETERS, JCH
DOI：
10.1016/s0005-2728(89)80347-0
发表时间：
1989-08-03
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
PORRA, RJ;THOMPSON, WA;KRIEDEMANN, PE
通讯作者：
KRIEDEMANN, PE
影响因子：
7.4
作者：
KOLBER, Z;ZEHR, J;FALKOWSKI, P
通讯作者：
FALKOWSKI, P
影响因子：
2.7
作者：
Juneau, P;Popovic, R
通讯作者：
Popovic, R