Measuring aptamer equilibria using gradient micro free flow electrophoresis.
Measuring aptamer equilibria using gradient micro free flow electrophoresis.
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DOI：
10.1021/ac902877v
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发表时间：
2010-05-01
影响因子：
7.4
通讯作者：
Bowser, Michael T.
中科院分区：
文献类型：
--
作者：
Turgeon, Ryan T.;Fonslow, Bryan R.;Jing, Meng;Bowser, Michael T.
Gradient micro free flow electrophoresis (μFFE) was used to observe the equilibria of DNA aptamers with their targets (IgE or HIVRT) across a range of ligand concentrations. A continuous stream of aptamer was mixed online with an increasing concentration of target and introduced into the μFFE device, which separated ligand-aptamer complexes from the unbound aptamer. The continuous nature of μFFE allowed the equilibrium distribution of aptamer and complex to be measured at 300 discrete target concentrations within 5 minutes. This is a significant improvement in speed and precision over affinity capillary electrophoresis (ACE) assays. The dissociation constant of the aptamer-IgE complex was estimated to be 48± 3 nM. The high coverage across the range of ligand concentrations allowed complex stoichiometries of the aptamer-HIVRT complexes to be observed. Nearly continuous observation of the equilibrium distribution from 0 to 500 nM HIVRT revealed the presence of complexes with 3:1 (aptamer:HIVRT), 2:1 and 1:1 stoichiometries.
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影响因子：
2.9
作者：
HOFFSTETTERKUHN, S;KUHN, R;WAGNER, H
通讯作者：
WAGNER, H
影响因子：
7.4
作者：
Fonslow, Bryan R.;Barocas, Victor H.;Bowser, Michael T.
通讯作者：
Bowser, Michael T.
影响因子：
7.4
作者：
Kohlheyer, Dietrich;Eijkel, Jan C. T.;Schasfoort, Richard B. M.
通讯作者：
Schasfoort, Richard B. M.
DOI：
10.1073/pnas.74.3.869
发表时间：
1977-01-01
影响因子：
11.1
作者：
NEWMAN, SA;ROSSI, G;METZGER, H
通讯作者：
METZGER, H
影响因子：
6.1
作者：
Frost NW;Bowser MT
通讯作者：
Bowser MT