Metabolomics and pathway analyses to characterize metabolic alterations in pregnant dairy cows on D 17 and D 45 after AI.
Metabolomics and pathway analyses to characterize metabolic alterations in pregnant dairy cows on D 17 and D 45 after AI.
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代谢组学和途径分析表征妊娠奶牛在 AI 后第 17 天和第 45 天的代谢变化
DOI：
10.1038/s41598-018-23983-2
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发表时间：
2018-04-13
影响因子：
4.6
通讯作者：
Tao JZ
中科院分区：
文献类型：
--
作者：
Guo YS;Tao JZ
营养流向胚胎和胎盘对于怀孕期间的正常发育和生长至关重要。为了更好地了解定时人工授精（AI）后妊娠奶牛在D17和D45的总体变化，进行了代谢组学分析。采用HPLC-MS和多元统计分析方法研究了妊娠奶牛血浆中代谢物的变化。使用MetPA方法建立代谢网络的变化。在D17和D45发现了六种代谢途径的改变，包括α-亚麻酸代谢、甘油磷脂代谢、戊糖和葡萄糖醛酸相互转化、甘油脂质代谢、叶酸生物合成和酪氨酸代谢水平的变化。除这些途径外，在D45时有9条代谢途径发生明显改变.这些途径包括由叶酸引起的一碳库的变化;苯丙氨酸、酪氨酸和色氨酸生物合成;硫胺素代谢;泛酸和CoA生物合成;嘌呤代谢;肌醇磷酸代谢;氨基糖和核苷酸糖代谢;戊糖磷酸;和TCA途径。本研究中使用的代谢组学和网络方法相结合，产生了丰富的生物化学洞察可能的生物模块相关的奶牛早期妊娠。
Nutrient flow to the embryo and placenta is crucial for proper development and growth during pregnancy. In this study, a metabonomic analysis was undertaken to better understand global changes in pregnant dairy cows on D 17 and D 45 after timed artificial insemination (AI). Metabolic changes in the blood plasma of pregnant dairy cows were investigated using HPLC-MS and a multivariate statistical analysis. Changes in metabolic networks were established using the MetPA method. Alterations in six metabolic pathways were found on D 17 and D 45, including variations in the level of alpha-linolenic acid metabolism, glycerophospholipid metabolism, pentose and glucuronate interconversions, glycerolipid metabolism, folate biosynthesis, and tyrosine metabolism. In addition to these pathways, 9 metabolic pathways were markedly altered on D 45. These pathways included changes in the one-carbon pool caused by folate; phenylalanine, tyrosine and tryptophan biosynthesis; thiamine metabolism; pantothenate and CoA biosynthesis; purine metabolism; inositol phosphate metabolism; amino sugar and nucleotide sugar metabolism; pentose phosphate; and the TCA pathway. The combination of metabonomics and network methods used in this study generated rich biochemical insight into possible biological modules related to early pregnancy in dairy cows.
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影响因子：
2.8
作者：
Binelli, M;Thatcher, WW;Baruselli, PS
通讯作者：
Baruselli, PS
影响因子：
5.8
作者：
Kankainen, Matti;Gopalacharyulu, Peddinti;Oresic, Matej
通讯作者：
Oresic, Matej
影响因子：
1.8
作者：
Nicholson, JK;Lindon, JC;Holmes, E
通讯作者：
Holmes, E
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
3.7
作者：
Abbasi, Alireza;Hossain, Liaquat;Leydesdorff, Loet
通讯作者：
Leydesdorff, Loet