SARS-CoV-2 and other respiratory pathogens are detected in continuous air samples from congregate settings.
SARS-CoV-2 and other respiratory pathogens are detected in continuous air samples from congregate settings.
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DOI：
10.1038/s41467-022-32406-w
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发表时间：
2022-08-11
影响因子：
16.6
通讯作者：
O'Connor, Shelby L.
中科院分区：
文献类型：
--
作者：
Ramuta, Mitchell D.;Newman, Christina M.;Brakefield, Savannah F.;Stauss, Miranda R.;Wiseman, Roger W.;Kita-Yarbro, Amanda;O'Connor, Eli J.;Dahal, Neeti;Lim, Ailam;Poulsen, Keith P.;Safdar, Nasia;Marx, John A.;Accola, Molly A.;Rehrauer, William M.;Zimmer, Julia A.;Khubbar, Manjeet;Beversdorf, Lucas J.;Boehm, Emma C.;Castaneda, David;Rushford, Clayton;Gregory, Devon A.;Yao, Joseph D.;Bhattacharyya, Sanjib;Johnson, Marc C.;Aliota, Matthew T.;Friedrich, Thomas C.;O'Connor, David H.;O'Connor, Shelby L.
在SARS-CoV-2出现两年后，仍然需要更好的方法来评估聚集空间的传播风险。我们部署了主动空气采样器，以监测威斯康星州和明尼苏达州上中西部社区的实际环境中SARS-CoV-2的存在。在29周的时间里，我们从15个聚集地收集了527个空气样本。我们检测了106个SARS-CoV-2病毒RNA阳性的样本，表明SARS-CoV-2可以在从各种真实环境中收集的连续空气样本中检测到。我们扩大了空气监测的效用，以检测40种其他呼吸道病原体。监测数据显示，SARS-CoV-2和甲型流感病毒检测的时间和地点存在差异。此外，我们从空气样本中获得了SARS-CoV-2基因组序列，以确定变异谱系。总的来说，这表明空气采样是一种可扩展的高通量监测工具，可与其他方法结合使用，用于检测聚集环境中的呼吸道病原体。空气监测提供了一种潜在的监测空气传播病原体的手段，而无需单独取样。在这里，作者在美国15个社区环境中进行了29周的连续空气采样，并证明了其用于SARS-CoV-2和其他呼吸道病原体常规检测的可行性。
Two years after the emergence of SARS-CoV-2, there is still a need for better ways to assess the risk of transmission in congregate spaces. We deployed active air samplers to monitor the presence of SARS-CoV-2 in real-world settings across communities in the Upper Midwestern states of Wisconsin and Minnesota. Over 29 weeks, we collected 527 air samples from 15 congregate settings. We detected 106 samples that were positive for SARS-CoV-2 viral RNA, demonstrating that SARS-CoV-2 can be detected in continuous air samples collected from a variety of real-world settings. We expanded the utility of air surveillance to test for 40 other respiratory pathogens. Surveillance data revealed differences in timing and location of SARS-CoV-2 and influenza A virus detection. In addition, we obtained SARS-CoV-2 genome sequences from air samples to identify variant lineages. Collectively, this shows air sampling is a scalable, high throughput surveillance tool that could be used in conjunction with other methods for detecting respiratory pathogens in congregate settings. Air surveillance offers a potential means of monitoring airborne pathogens without the need for individual sampling. Here, the authors perform continuous air sampling in 15 community settings in the US for 29 weeks and demonstrate its feasibility for routine detection of SARS-CoV-2 and other respiratory pathogens.
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DOI：
10.1056/nejmoa2119451
发表时间：
2022-04-21
期刊：
The New England journal of medicine
影响因子：
--
作者：
Andrews N;Stowe J;Kirsebom F;Toffa S;Rickeard T;Gallagher E;Gower C;Kall M;Groves N;O'Connell AM;Simons D;Blomquist PB;Zaidi A;Nash S;Iwani Binti Abdul Aziz N;Thelwall S;Dabrera G;Myers R;Amirthalingam G;Gharbia S;Barrett JC;Elson R;Ladhani SN;Ferguson N;Zambon M;Campbell CNJ;Brown K;Hopkins S;Chand M;Ramsay M;Lopez Bernal J
通讯作者：
Lopez Bernal J
影响因子：
5.1
作者：
Grigsby-Toussaint, Diana S.;Shin, Jong Cheol;Jones, Antwan
通讯作者：
Jones, Antwan
影响因子：
5.8
作者：
Fabian P;McDevitt JJ;Houseman EA;Milton DK
通讯作者：
Milton DK
DOI：
10.15585/mmwr.mm6946e1
发表时间：
2020-11-20
期刊：
MMWR. Morbidity and mortality weekly report
影响因子：
--
作者：
Denny TN;Andrews L;Bonsignori M;Cavanaugh K;Datto MB;Deckard A;DeMarco CT;DeNaeyer N;Epling CA;Gurley T;Haase SB;Hallberg C;Harer J;Kneifel CL;Lee MJ;Louzao R;Moody MA;Moore Z;Polage CR;Puglin J;Spotts PH;Vaughn JA;Wolfe CR
通讯作者：
Wolfe CR
影响因子：
3.4
作者：
Cordes, Jack;Castro, Marcia C.
通讯作者：
Castro, Marcia C.