Airborne influenza virus detection with four aerosol samplers using molecular and infectivity assays: considerations for a new infectious virus aerosol sampler.
Airborne influenza virus detection with four aerosol samplers using molecular and infectivity assays: considerations for a new infectious virus aerosol sampler.
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DOI：
10.1111/j.1600-0668.2009.00609.x
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发表时间：
2009-10
期刊：
影响因子：
5.8
通讯作者：
Milton DK
中科院分区：
文献类型：
--
作者：
Fabian P;McDevitt JJ;Houseman EA;Milton DK
作为进行空气中流感传播研究的第一步，我们比较了SKC生物采样器、紧凑型级联撞击器（CCI）、特氟隆过滤器和明胶过滤器在一次通过气溶胶室中收集气溶胶化流感病毒的收集性能。使用荧光病灶测定法测定流感病毒感染性，并使用定量PCR测量流感病毒核酸（源自活病毒和非活病毒）。结果表明，SKC生物采样器回收和保存流感病毒感染性的效果比其他采样器好得多-与生物采样器相比，CCI、Teflon和明胶过滤器仅回收了7-22%的感染性病毒。SKC生物采样器、明胶和Teflon过滤器的总病毒收集量无显著差异，但CCI中的病毒收集量显著较低。这项研究的结果表明，需要一种新的采样器进行病毒气溶胶采样，因为市售采样器不能有效地收集和保存病毒感染性。新采样器的应用包括空气传播疾病的研究和生物恐怖主义监测。新采样器的设计参数包括对细颗粒和液体采样介质的高收集效率，以保持感染性。
As a first step in conducting studies of airborne influenza transmission, we compared the collection performance of an SKC Biosampler, a compact cascade impactor (CCI), Teflon filters, and gelatin filters by collecting aerosolized influenza virus in a one-pass aerosol chamber. Influenza virus infectivity was determined using a fluorescent focus assay and influenza virus nucleic acid (originating from viable and non-viable viruses) was measured using quantitative PCR. The results showed that the SKC Biosampler recovered and preserved influenza virus infectivity much better than the other samplers – the CCI, Teflon, and gelatin filters recovered only 7–22% of infectious viruses compared with the Biosampler. Total virus collection was not significantly different among the SKC Biosampler, the gelatin, and Teflon filters, but was significantly lower in the CCI. Results from this study show that a new sampler is needed for virus aerosol sampling, as commercially available samplers do not efficiently collect and conserve virus infectivity. Applications for a new sampler include studies of airborne disease transmission and bioterrorism monitoring. Design parameters for a new sampler include high collection efficiency for fine particles and liquid sampling media to preserve infectivity.
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影响因子：
6.4
作者：
Booth, TF;Kournikakis, B;Plummer, F
通讯作者：
Plummer, F
影响因子：
5.8
作者：
Hartshorn, Kevan L.;White, Mitchell R.;Holmskov, Uffe
通讯作者：
Holmskov, Uffe
影响因子：
4.4
作者：
Agranovski, IE;Safatov, AS;Agranovski, V
通讯作者：
Agranovski, V
DOI：
10.1073/pnas.0408159101
发表时间：
2004-12-14
影响因子：
11.1
作者：
Edwards, DA;Man, JC;Scheuch, G
通讯作者：
Scheuch, G
影响因子：
4.5
作者：
Lee, SJ;Demokritou, P;Koutrakis, P
通讯作者：
Koutrakis, P