Effect of brominated furanones on the formation of biofilm by Escherichia coli on polyvinyl chloride materials.
Effect of brominated furanones on the formation of biofilm by Escherichia coli on polyvinyl chloride materials.
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溴化呋喃酮对聚氯乙烯材料上大肠杆菌生物膜形成的影响
DOI：
10.1007/s12013-013-9578-8
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发表时间：
2013
影响因子：
2.6
通讯作者：
Yujie, Lei
中科院分区：
文献类型：
--
作者：
Lianhua, Ye;Yunchao, Huang;Geng, Xu;Youquang, Zhou;Guangqiang, Zhao;Yujie, Lei
为研究溴代呋喃酮对大肠杆菌在聚氯乙烯（PVC）材料上生物被膜（BF）形成的影响，为材料表面改性以从临床角度预防以生物材料为中心的感染提供新途径。将三种具有代表性化学结构的溴代呋喃酮（呋喃酮 - 1（3,4 - 二溴 - 5 - 羟基 - 呋喃酮）、呋喃酮 - 2（4 - 溴 - 5 -（4 - 甲氧基苯基） - 3 -（甲氨基） - 呋喃酮）和呋喃酮 - 3（3,4 - 二溴 - 5,5 - 二甲氧基苯基 - 2（5H） - 呋喃酮））溶解于乙醇后，分别涂覆在不同的PVC材料（1×1厘米）表面。将表面改性后的PVC材料与大肠杆菌共同培养，对照组使用75%乙醇处理过的PVC材料。培养时间分别为6小时、12小时、18小时和24小时。通过共聚焦激光扫描显微镜（CLSM）测定PVC材料上细菌生物被膜的厚度和单位面积细菌群落数量，利用扫描电子显微镜（SEM）观察细菌生物被膜形成的表面结构。CLSM结果表明，在所有时间点，呋喃酮 - 3处理的PVC材料上细菌生物被膜的厚度和单位面积细菌群落数量均显著低于对照组（P < 0.05），而呋喃酮 - 1组和呋喃酮 - 2组与对照组之间差异不显著（P > 0.05）。SEM结果显示，培养6小时后，呋喃酮 - 3处理的PVC材料表面细菌附着数量低于对照组。培养至18小时时，对照PVC材料表面已完全形成生物被膜结构，然而，呋喃酮 - 3处理的PVC材料表面未形成明显的生物被膜。不同溴代呋喃酮对PVC材料表面大肠杆菌生物被膜形成的影响存在差异，呋喃酮 - 3可抑制大肠杆菌在PVC材料表面生物被膜的形成。
To study the influence of brominated furanones on the biofilm (BF) formation by Escherichia coli (E. coli) on polyvinyl chloride (PVC) material, and to provide new ways of surface modification of materials to clinically prevent biomaterial centered infection. Three brominated furanones, dissolved in ethanol, furanone-1(3,4-dibromo-5-hydroxyl-furanone), furanone-2(4-bromo-5-(4-methoxypheny)-3-(methylamino)-furanone), and furanone-3(3,4-dibromo-5,5-dimethoxypheny-2(5H)-furanone) with representative chemical structure, were coated on the surfaces of separate PVC materials (1 × 1 cm), respectively. The surface-modified PVC materials were incubated with E. coli and for controls, 75 % ethanol-treated PVC materials were used. This treatment played as control group. The cultivation incubations were for 6, 12, 18, and 24 h. The thickness of bacterial BF and bacterial community quantity unit area on the PVC materials was determined by confocal laser scanning microscopy (CLSM), and the surface structure of bacterial BF formation was examined by scanning electron microscopy (SEM). The results of CLSM indicated the thickness of bacterial BF and bacterial community quantity unit area on PVC materials treated with furanone-3 were significantly lower than that of control at all time points (P < 0.05), whereas, the differences between furanone-1 and furanone-2 groups and control group were not significantly different (P > 0.05). The results of SEM indicated that after 6 h incubation, the quantity of bacterial attachment to the surface of PVC material treated with furanone-3 was lower than the control group. By 18 h incubation there was completely formed BF structure on the surface of control PVC material. However, there was no significant BF formation on the surface of PVC material treated with furanone-3. The impact of different brominated furanones on SA biofilm formation on the surface of PVC materials are different, furanone-3 can inhibit E. coli biofilm formation on the surface of PVC material.
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影响因子：
3
作者：
Benedetti, Paolo;Pellizzer, Giampietro;Sefton, Armine
通讯作者：
Sefton, Armine
影响因子：
3
作者：
Teasdale, Margaret E.;Donovan, Kellye A.;Rowley, David C.
通讯作者：
Rowley, David C.
影响因子：
4.3
作者：
Faggion, Clovis Mariano, Jr.;Chambrone, Leandro;Tu, Yu-Kang
通讯作者：
Tu, Yu-Kang
DOI：
10.1073/pnas.0502890102
发表时间：
2006-02-14
影响因子：
11.1
作者：
Yang, S;Lopez, CR;Zechiedrich, EL
通讯作者：
Zechiedrich, EL
影响因子：
64.8
作者：
Chen, X;Schauder, S;Hughson, FM
通讯作者：
Hughson, FM