Two types of galactosylated fucose motifs are present on N-glycans of Haemonchus contortus.
Two types of galactosylated fucose motifs are present on N-glycans of Haemonchus contortus.
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DOI：
10.1093/glycob/cwv015
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发表时间：
2015-06
期刊：
影响因子：
4.3
通讯作者：
Wilson IB
中科院分区：
文献类型：
--
作者：
Paschinger K;Wilson IB
来自捻转血矛线虫（一种绵羊和牛的寄生虫）的N-聚糖是首次被描述为具有与N，N '-二乙酰壳二糖基核心相关的多达三个岩藻糖残基，两个在还原末端近端GlcNAc残基上，一个在远端核心GlcNAc残基上。假设来自该微生物的具有三个己糖残基的截短聚糖的组成为Man 3GlcNAc 2Fuc 1 -3。在这项研究中，我们已经进行了HPLC和MALDI-TOF MS/MS结合选择性的digestion的N-聚糖从血矛线虫。主要的三岩藻糖基化Hex 3 HexNAc 2Fuc 3聚糖不仅被α 1，6-岩藻糖修饰，而且被近端核心α 1，3-岩藻糖和半乳糖基化远端α 1，3-岩藻糖修饰;因此，只有两个己糖残基是甘露糖。其他N-聚糖显示核心α 1，6-岩藻糖的半乳糖基化、触角岩藻糖基化或磷酸胆碱修饰。因此，血矛线虫的N-聚糖含有许多在其他寄生虫中也发现的潜在免疫原性聚糖表位，并且我们提出的结构与之前定义的线虫糖基转移酶的特异性一致，因为我们表明远端岩藻糖基化和α 1，6-甘露糖的存在显然是相互排斥的。因此，这些数据对于在制备作为疫苗候选物的重组蛋白中能够模拟血矛线虫型N-聚糖的工程化细胞系是重要的。
N-glycans from the nematode Haemonchus contortus (barber pole worm), a parasite of sheep and cattle, were the first to be described to possess up to three fucose residues associated with the N,N’-diacetylchitobiosyl core, two being on the reducing-terminal proximal GlcNAc and one on the distal core GlcNAc residue. The assumption was that truncated glycans from this organism with three hexose residues have the composition Man3GlcNAc2Fuc1-3. In this study we have performed HPLC and MALDI-TOF MS/MS in combination with selected digestions of N-glycans from Haemonchus. A dominant trifucosylated Hex3HexNAc2Fuc3 glycan was modified not only with α1,6-fucose, but also with a proximal core α1,3-fucose and a galactosylated distal α1,3-fucose; thereby, only two of the hexose residues were mannose. Other N-glycans displayed galactosylation of the core α1,6-fucose, antennal fucosylation or modification with phosphorylcholine. Thus, the N-glycans of Haemonchus contain a number of potentially immunogenic glycan epitopes also found in other parasites and our proposed structures are in line with the previously-defined specificity of nematode glycosyltransferases as we show distal fucosylation and the presence of an α1,6-mannose are apparently mutually exclusive. These data are thereby of importance for engineering cell lines capable of mimicking Haemonchus-type N-glycans in the preparation of recombinant proteins as vaccine candidates.
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影响因子：
--
作者：
ALBERS, GAA;GRAY, GD;BARKER, JSF
通讯作者：
BARKER, JSF
影响因子：
6.7
作者：
Schubert M;Bleuler-Martinez S;Butschi A;Wälti MA;Egloff P;Stutz K;Yan S;Collot M;Mallet JM;Wilson IB;Hengartner MO;Aebi M;Allain FH;Künzler M
通讯作者：
Künzler M
影响因子：
2.6
作者：
Hein, WR;Harrison, GBL
通讯作者：
Harrison, GBL
影响因子：
4.3
作者：
Dragosits M;Yan S;Razzazi-Fazeli E;Wilson IB;Rendic D
通讯作者：
Rendic D
影响因子：
2.9
作者：
TOMIYA, N;AWAYA, J;TAKAHASHI, N
通讯作者：
TAKAHASHI, N