The Protein Level of Rev1, a TLS Polymerase in Fission Yeast, Is Strictly Regulated during the Cell Cycle and after DNA Damage.
The Protein Level of Rev1, a TLS Polymerase in Fission Yeast, Is Strictly Regulated during the Cell Cycle and after DNA Damage.
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DOI：
10.1371/journal.pone.0130000
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Hanaoka F
中科院分区：
文献类型：
--
作者：
Uchiyama M;Terunuma J;Hanaoka F
当模板DNA受损时，转录DNA合成提供了另一种DNA复制机制。在裂殖酵母中，Eso 1（polη）、Kpa 1/DinB（polκ）、Rev 1和Pol β（Rev 3和Rev 7的复合物）已被鉴定为跨损伤合成聚合酶。这些聚合酶的酶特性和蛋白质-蛋白质相互作用已被深入研究，然而，这些蛋白质在细胞周期中如何调控仍不清楚。因此，我们研究了跨损伤聚合酶的细胞周期振荡。有趣的是，Rev 1的蛋白水平在G1期达到峰值，然后在进入S期时急剧下降，这种调节依赖于蛋白酶体。温度敏感的蛋白酶体突变体，如mts 2-U31和mts 3-U32，稳定Rev 1蛋白时，温度转移到限制性条件。此外，SCF泛素连接酶复合物的亚基pop 1或pop 2的缺失上调Rev 1蛋白水平。除了在细胞周期中的这些作用外，我们还观察到Rev 1蛋白在DNA损伤后的上调。当检测点蛋白rad 3被删除或Rev 1启动子被组成型启动子取代时，这种上调被取消。从这些结果，我们假设，translesion DNA合成严格控制通过Rev 1蛋白水平，以避免不必要的诱变。
Translesion DNA synthesis provides an alternative DNA replication mechanism when template DNA is damaged. In fission yeast, Eso1 (polη), Kpa1/DinB (polκ), Rev1, and Polζ (a complex of Rev3 and Rev7) have been identified as translesion synthesis polymerases. The enzymatic characteristics and protein-protein interactions of these polymerases have been intensively characterized; however, how these proteins are regulated during the cell cycle remains unclear. Therefore, we examined the cell cycle oscillation of translesion polymerases. Interestingly, the protein levels of Rev1 peaked during G1 phase and then decreased dramatically at the entry of S phase; this regulation was dependent on the proteasome. Temperature-sensitive proteasome mutants, such as mts2-U31 and mts3-U32, stabilized Rev1 protein when the temperature was shifted to the restrictive condition. In addition, deletion of pop1 or pop2, subunits of SCF ubiquitin ligase complexes, upregulated Rev1 protein levels. Besides these effects during the cell cycle, we also observed upregulation of Rev1 protein upon DNA damage. This upregulation was abolished when rad3, a checkpoint protein, was deleted or when the Rev1 promoter was replaced with a constitutive promoter. From these results, we hypothesize that translesion DNA synthesis is strictly controlled through Rev1 protein levels in order to avoid unwanted mutagenesis.
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影响因子：
7.2
作者：
Goodman MF;Woodgate R
通讯作者：
Woodgate R
DOI：
10.1073/pnas.0902175106
发表时间：
2009-06-16
影响因子：
11.1
作者：
Acharya, Narottam;Johnson, Robert E.;Prakash, Satya
通讯作者：
Prakash, Satya
影响因子：
16
作者：
Guo, Caixia;Sonoda, Eiichiro;Friedberg, Errol C.
通讯作者：
Friedberg, Errol C.
影响因子：
64.8
作者：
Jarosz, DF;Godoy, VG;Walker, GC
通讯作者：
Walker, GC
影响因子：
5.3
作者：
Hicks, J. Kevin;Chute, Colleen L.;Canman, Christine E.
通讯作者：
Canman, Christine E.