Cotton Late Embryogenesis Abundant (LEA2) Genes Promote Root Growth and Confer Drought Stress Tolerance in Transgenic Arabidopsis thaliana.
Cotton Late Embryogenesis Abundant (LEA2) Genes Promote Root Growth and Confer Drought Stress Tolerance in Transgenic Arabidopsis thaliana.
复制标题
棉花晚期胚胎发生丰富 (LEA2) 基因促进转基因拟南芥根部生长并赋予干旱胁迫耐受性
DOI：
10.1534/g3.118.200423
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发表时间：
2018-07-31
期刊：
影响因子：
--
通讯作者：
Liu F
中科院分区：
文献类型：
--
作者：
Magwanga RO;Lu P;Kirungu JN;Dong Q;Hu Y;Zhou Z;Cai X;Wang X;Hou Y;Wang K;Liu F
胚胎发生后期丰富蛋白（LEA）在植物抗旱性中起着关键作用。在本研究中，分别从毛毛藓、树藓和雷蒙地中鉴定出157、85和89个候选LEA2蛋白。将LEA2基因分为6组，分别为1 ~ 6组。系统发育树分析显示棉花基因组中存在同源基因对。除Y、K和S基序外，棉花特异的LEA2基序还有E、R和D基序。这些基因分布在所有染色体上。LEA2s在非极性脂肪族氨基酸残基中高度富集，其中亮氨酸的比例最高，为9.1%。已知ghr-miR827a/b/c/d和ghr-miR164靶向的许多基因对干旱胁迫有反应。检测了各种胁迫响应调控元件、aba响应元件（ABRE）、干旱响应元件（DRE/CRT）、MYBS和低温响应元件（LTRE）。大部分基因在叶片和根中高度表达，是受水分亏缺影响最大的主要器官。毛毛绒毛毛绒的表达水平比毛绒毛毛绒高得多。耐受性基因型诱导更多LEA2基因的能力更强。转化基因Cot_AD24498的过表达表明，LEA2基因参与促进根系生长，从而使其具有耐旱性。因此，我们推测在LEA2基因中，Cot_AD24498、CotAD_20020、cotad__21924和CotAD_59405可能是旱棉干旱胁迫下具有重要功能的候选基因。转化后的拟南芥植株对干旱胁迫的耐受性高于野生型。干旱胁迫下，转化系叶片抗氧化剂、过氧化氢酶（CAT）、过氧化物酶（POD）和超氧化物歧化酶（SOD）积累量显著增加，根长增加，抗氧化剂、过氧化氢（H2O2）和丙二醛（MDA）浓度显著降低。本研究提供了棉花LEA2蛋白的全面分析，为育种者利用这些基因开发耐旱基因型奠定了基础。
Late embryogenesis abundant (LEA) proteins play key roles in plant drought tolerance. In this study, 157, 85 and 89 candidate LEA2 proteins were identified in G. hirsutum, G. arboreum and G. raimondii respectively. LEA2 genes were classified into 6 groups, designated as group 1 to 6. Phylogenetic tree analysis revealed orthologous gene pairs within the cotton genome. The cotton specific LEA2 motifs identified were E, R and D in addition to Y, K and S motifs. The genes were distributed on all chromosomes. LEA2s were found to be highly enriched in non-polar, aliphatic amino acid residues, with leucine being the highest, 9.1% in proportion. The miRNA, ghr-miR827a/b/c/d and ghr-miR164 targeted many genes are known to be drought stress responsive. Various stress-responsive regulatory elements, ABA-responsive element (ABRE), Drought-responsive Element (DRE/CRT), MYBS and low-temperature-responsive element (LTRE) were detected. Most genes were highly expressed in leaves and roots, being the primary organs greatly affected by water deficit. The expression levels were much higher in G. tomentosum as opposed to G. hirsutum. The tolerant genotype had higher capacity to induce more of LEA2 genes. Over expression of the transformed gene Cot_AD24498 showed that the LEA2 genes are involved in promoting root growth and in turn confers drought stress tolerance. We therefore infer that Cot_AD24498, CotAD_20020, CotAD_21924 and CotAD_59405 could be the candidate genes with profound functions under drought stress in upland cotton among the LEA2 genes. The transformed Arabidopsis plants showed higher tolerance levels to drought stress compared to the wild types. There was significant increase in antioxidants, catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) accumulation, increased root length and significant reduction in oxidants, Hydrogen peroxide (H2O2) and malondialdehyde (MDA) concentrations in the leaves of transformed lines under drought stress condition. This study provides comprehensive analysis of LEA2 proteins in cotton thus forms primary foundation for breeders to utilize these genes in developing drought tolerant genotypes.
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影响因子：
14.9
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