Molecular imaging detects impairment in the retrograde axonal transport mechanism after radiation-induced spinal cord injury.
Molecular imaging detects impairment in the retrograde axonal transport mechanism after radiation-induced spinal cord injury.
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DOI：
10.1007/s11307-013-0713-0
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发表时间：
2014-08
影响因子：
3.1
通讯作者：
Schellingerhout, Dawid
中科院分区：
文献类型：
--
作者：
LeRoux, Lucia G.;Bredow, Sebastian;Grosshans, David;Schellingerhout, Dawid
关键词：
The goal of this study was to determine whether molecular imaging of retrograde axonal transport is a suitable technique to detect changes in the spinal cord in response to radiation injury. The lower thoracic spinal cords of adult female BALB/c mice were irradiated with single doses of 2, 10, or 80 Gy. An optical imaging method was used to observe the migration of the fluorescently labeled non-toxic C-fragment of tetanus toxin (TTc) from an injection site in the calf muscles to the spinal cord. Changes in migration patterns compared to baseline and controls allowed assessment of radiation-induced alterations in the retrograde neuronal axonal transport mechanism. Subsequently, tissues were harvested and histological examination of the spinal cords performed. Transport of TTc in the thoracic spinal cord was impaired in a dose-dependent manner. Transport was significantly decreased by 16 d in animals exposed to either 10 or 80 Gy, while animals exposed to 2 Gy were affected only minimally. Further, animals exposed to the highest dose also experienced significant weight loss by 9 d and developed posterior paralysis by 45 d. Marked histological changes including vacuolization and white matter necrosis were observed in radiated cords after 30 d for mice exposed to 80 Gy. Radiation of the spinal cord induces dose-dependent changes in retrograde axonal transport, which can be monitored by molecular imaging. This approach suggests a novel diagnostic modality to assess nerve injury and monitor therapeutic interventions.
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影响因子：
6.2
作者：
Garg, Amit K.;Shiu, Almon S.;Yang, James;Wang, Xin-Shelley;Allen, Pamela;Brown, Barry W.;Grossman, Patricia;Frija, Erik K.;McAleer, Mary Frances;Azeem, Syed;Brown, Paul D.;Rhines, Laurence D.;Chang, Eric L.
通讯作者：
Chang, Eric L.
DOI：
10.1016/0360-3016(92)90982-n
发表时间：
1992-01-01
影响因子：
7
作者：
LO, YC;MCBRIDE, WH;WITHERS, HR
通讯作者：
WITHERS, HR
DOI：
10.1016/j.ijrobp.2010.07.1979
发表时间：
2011-01-01
影响因子：
7
作者：
Medin, Paul M.;Foster, Ryan D.;Solberg, Timothy D.
通讯作者：
Solberg, Timothy D.
影响因子：
3.7
作者：
Schellingerhout, Dawid;LeRoux, Lucia G.;Bredow, Sebastian
通讯作者：
Bredow, Sebastian
DOI：
10.1016/j.ijrobp.2011.08.030
发表时间：
2012-07-01
影响因子：
7
作者：
Medin, Paul M.;Foster, Ryan D.;van der Kogel, Albert J.;Sayre, James W.;McBride, William H.;Solberg, Timothy D.
通讯作者：
Solberg, Timothy D.