Differential expression and processing of transforming growth factor beta induced protein (TGFBIp) in the normal human cornea during postnatal development and aging.
Differential expression and processing of transforming growth factor beta induced protein (TGFBIp) in the normal human cornea during postnatal development and aging.
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DOI：
10.1016/j.exer.2009.09.011
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发表时间：
2010-01
影响因子：
3.4
通讯作者：
Enghild JJ
中科院分区：
文献类型：
--
作者：
Karring H;Runager K;Valnickova Z;Thøgersen IB;Møller-Pedersen T;Klintworth GK;Enghild JJ
转化生长因子β诱导蛋白（TGF β 1 β，也称为角膜上皮素）是角膜中丰富的细胞外基质蛋白。本研究的目的是确定TGF β 1 β在正常人角膜出生后发育和衰老过程中的表达和加工。通过免疫印迹法检测和定量来自年龄范围从6个月至86岁的个体的角膜中的TGF β 1 β。角膜中的TGF β 1 β水平在6至14岁之间增加约30%，成人角膜每毫克湿组织含有0.7-0.8 μg TGF β 1 β。角膜提取物的二维（2-D）免疫印迹显示由不同的低分子量TGF β 1 β亚型（30-60 kDa）形成的特征性“之字形”图案。然而，不同亚型的相对丰度在婴儿角膜（< 1岁）和儿童/成人角膜（> 6岁）之间是不同的。基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）数据的TGF β 1 β亚型分离的大2-D凝胶显示，TGF β 1 β是蛋白水解加工的N-末端。这一观察结果得到了计算机2-D凝胶电泳的支持，显示来自成熟TGF β 1 β N-末端的连续蛋白水解修剪事件产生TGF β 1 β同种型，其在通过2-D聚丙烯酰胺凝胶电泳（PAGE）分离时形成类似的“之字形”模式。这项研究表明，在人类中，TGF β 1 β在成熟角膜中比在发育中的角膜中更丰富，并且TGF β 1 β的加工在角膜的出生后发育期间发生变化。此外，TGF β 1 β似乎在正常人角膜中以高度协调的方式降解，所得的C末端片段保留在角膜中。角膜TGF β 1 β表达和加工的年龄相关性变化表明TGF β 1 β可能在角膜的出生后发育和成熟中起作用。此外，这些观察结果可能与转化生长因子β诱导基因（TGFBI）突变引起的营养不良中突变TGF β 1 β沉积在角膜中的年龄以及角膜蛋白沉积的机制有关。
Transforming growth factor beta induced protein (TGFBIp, also named keratoepithelin) is an extracellular matrix protein abundant in the cornea. The purpose of this study was to determine the expression and processing of TGFBIp in the normal human cornea during postnatal development and aging. TGFBIp in corneas from individuals ranging from six months to 86 years of age was detected and quantified by immunoblotting. The level of TGFBIp in the cornea increases about 30% between 6 and 14 years of age, and adult corneas contain 0.7–0.8 µg TGFBIp per mg wet tissue. Two-dimentional (2-D) immunoblots of the corneal extracts showed a characteristic “zig-zag” pattern formed by different lower-molecular mass TGFBIp isoforms (30–60 kDa). However, the relative abundance of the different isoforms was different between infant corneas (< 1 year) and the child/adult corneas (> 6 years). Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) data of TGFBIp isoforms separated on large 2-D gels show that TGFBIp is proteolytically processed from the N-terminus. This observation was supported by in silico 2-D gel electrophoresis showing that sequential proteolytical trimming events from the N-terminus of mature TGFBIp generate TGFBIp isoforms which form a similar “zig-zag” pattern when separated by 2-D polyacrylamide gel electrophoresis (PAGE). This study shows that in humans TGFBIp is more abundant in mature corneas than in the developing cornea and that the processing of TGFBIp changes during postnatal development of the cornea. In addition, TGFBIp appears to be degraded in a highly orchestrated manner in the normal human cornea with the resulting C-terminal fragments being retained in the cornea. The age-related changes in the expression and processing of corneal TGFBIp suggests that TGFBIp may play a role in the postnatal development and maturation of the cornea. Furthermore, these observations may be relevant to the age at which mutant TGFBIp deposits in the cornea in those dystrophies caused by mutations in the transforming growth factor beta induced gene (TGFBI) as well as the mechanisms of corneal protein deposition.
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影响因子：
3.9
作者：
Kannabiran, Chitra;Klintworth, Gordon K.
通讯作者：
Klintworth, Gordon K.
DOI：
10.1016/s0006-291x(02)00576-4
发表时间：
2002-06-28
影响因子：
3.1
作者：
Bae, JS;Lee, SH;Kim, IS
通讯作者：
Kim, IS
影响因子：
2.9
作者：
Andersen, RB;Karring, H;Enghild, JJ
通讯作者：
Enghild, JJ
影响因子：
4.2
作者：
Hussein, MAW;Paysse, EA;Wilhelmus, KR
通讯作者：
Wilhelmus, KR
影响因子：
4.2
作者：
Dighiero, P;Drunat, S;Valleix, S
通讯作者：
Valleix, S