Stimulation of transcription factor binding and histone displacement by nucleosome assembly protein 1 and nucleoplasmin requires disruption of the histone octamer
Stimulation of transcription factor binding and histone displacement by nucleosome assembly protein 1 and nucleoplasmin requires disruption of the histone octamer
复制标题
核小体组装蛋白 1 和核蓝蛋白刺激转录因子结合和组蛋白置换需要破坏组蛋白八聚体
DOI：
--
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发表时间：
1995
影响因子：
5.3
通讯作者：
Jerry L. Workman
中科院分区：
文献类型：
--
作者：
P. P. Walter;Tom Owen;Jacques Côté;Jerry L. Workman
To investigate the mechanisms by which transcription factors invade nucleosomal DNA and replace histones at control elements, we have examined the response of the histone octamer to transcription factor binding in the presence of histone-binding proteins (i.e., nucleosome assembly factors). We found that yeast nucleosome assembly protein 1 (NAP-1) stimulated transcription factor binding and nucleosome displacement in a manner similar to that of nucleoplasmin. In addition, disruption of the histone octamer was required both for the stimulation of transcription factor binding to nucleosomal DNA and for transcription factor-induced nucleosome displacement mediated by nucleoplasmin or NAP-1. While NAP-1 and nucleoplasmin stimulated the binding of a fusion protein (GAL4-AH) to control nucleosome cores, this stimulation was lost upon covalent histone-histone cross-linking within the histone octamers. In addition, both NAP-1 and nucleoplasmin were able to mediate histone displacement upon the binding of five GAL4-AH dimers to control nucleosome cores; however, this activity was also forfeited when the histone octamers were cross-linked. These data indicate that octamer disruption is required for both stimulation of factor binding and factor-dependent histone displacement by nucleoplasmin and NAP-1. By contrast, transcription factor-induced histone transfer onto nonspecific competitor DNA did not require disruption of the histone octamer. Thus, histone displacement in this instance occurred by transfer of complete histone octamers, a mechanism distinct from that mediated by the histone-binding proteins nucleoplasmin and NAP-1.
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DOI：
10.1002/j.1460-2075.1991.tb08031.x
发表时间：
1991-04
期刊：
The EMBO Journal
影响因子：
--
作者：
Susan Smith;B. Stillman
通讯作者：
Susan Smith;B. Stillman
影响因子：
10.5
作者：
IZBAN, MG;LUSE, DS
通讯作者：
LUSE, DS
c-Myc 和 Max 与核小体 DNA 的差异结合。
影响因子：
5.3
作者：
Wechsler,DS;Papoulas,O;Dang,CV;Kingston,RE
通讯作者：
Kingston,RE
体外核小体组装过程中 II 类基因转录的控制。
影响因子：
--
作者：
Workman,JL;Taylor,IC;Kingston,RE;Roeder,RG
通讯作者：
Roeder,RG
影响因子：
56.9
作者：
COTE, J;QUINN, J;PETERSON, CL
通讯作者：
PETERSON, CL