Contrast-Enhanced Ultrasound Reveals Partial Perfusion Recovery After Hindlimb Ischemia as Opposed to Full Recovery by Laser Doppler Perfusion Imaging.
Contrast-Enhanced Ultrasound Reveals Partial Perfusion Recovery After Hindlimb Ischemia as Opposed to Full Recovery by Laser Doppler Perfusion Imaging.
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DOI：
10.1016/j.ultrasmedbio.2022.02.002
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发表时间：
2022-06
影响因子：
2.9
通讯作者：
French, Brent A.
中科院分区：
文献类型：
--
作者：
Becker, Alyssa B.;Chen, Lanlin;Ning, Bo;Hu, Song;Hossack, John A.;Klibanov, Alexander L.;Annex, Brian H.;French, Brent A.
小鼠模型在开发治疗外周动脉疾病（PAD）的新治疗方法中至关重要。尽管经过了数十年的研究和大量的临床试验，但现有疗法的疗效有限。这可能表明我们目前的动物模型和/或评估方法存在缺陷。我们通过比较激光多普勒灌注成像（LDPI，最常见的技术），对比增强超声（CEUS，新兴技术）和荧光微球（传统标准），在PAD小鼠模型中评价灌注测量方法。在基线、手术后1、4、7、14、28、60、90和150天通过LDPI和CEUS评估经历股动脉结扎的小鼠，以评价缺血后肢中的灌注恢复。手术后14天，通过荧光微球、LDPI和CEUS测量另外的小鼠。LDPI和CEUS显示了大致相似的灌注恢复趋势，直至术后7天。然而，到第14天，LDPI显示灌注完全恢复，而CEUS显示约50%的恢复，即使在5个月后也未能改善。与CEUS结果一致，术后第14天的荧光微球证实灌注恢复不完全。组织学和光声显微镜检查提供了持续血管异常的进一步证据。
Mouse models are critical in developing new therapeutic approaches to treat peripheral arterial disease (PAD). Despite decades of research and numerous clinical trials, the efficacy of available therapies is limited. This may suggest shortcomings in our current animal models and/or methods of assessment. We evaluated perfusion measurement methods in a mouse model of PAD by comparing laser Doppler perfusion imaging (LDPI, most common technique), contrast enhanced ultrasound (CEUS, emerging technique), and fluorescent microspheres (conventional standard). Mice undergoing a femoral artery ligation were assessed by LDPI and CEUS at baseline, 1, 4, 7, 14, 28, 60, 90, and 150 days post-surgery to evaluate perfusion recovery in the ischemic hindlimb. 14 days post-surgery, additional mice were measured by fluorescent microspheres, LDPI, and CEUS. LDPI and CEUS showed broadly similar trends of perfusion recovery until 7 days post-surgery. However, by day 14, LDPI indicated a full recovery of perfusion, whereas CEUS showed a ~50% recovery which failed to improve even after 5 months. In agreement with the CEUS results, fluorescent microspheres at day 14 post-surgery confirmed that perfusion recovery was incomplete. Histopathology and photoacoustic microscopy provided further evidence of sustained vascular abnormalities.
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影响因子：
168.9
作者：
Fowkes, F. Gerald R.;Rudan, Diana;Criqui, Michael H.
通讯作者：
Criqui, Michael H.
影响因子：
3.3
作者：
BUCKBERG, GD;LUCK, JC;FIXLER, DE
通讯作者：
FIXLER, DE
DOI：
10.1161/atvbaha.115.306506
发表时间：
2015-11-01
影响因子：
8.7
作者：
Cao, Wei J.;Rosenblat, Joshua D.;Leong-Poi, Howard
通讯作者：
Leong-Poi, Howard
影响因子：
20.1
作者：
Arpino, John-Michael;Nong, Zengxuan;Pickering, J. Geoffrey
通讯作者：
Pickering, J. Geoffrey
影响因子：
7.4
作者：
Jadert, Cecilia;Petersson, Joel;Phillipson, Mia
通讯作者：
Phillipson, Mia