Brain lipid-binding protein promotes proliferation and modulates cell cycle in C6 rat glioma cells.
Brain lipid-binding protein promotes proliferation and modulates cell cycle in C6 rat glioma cells.
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脑脂质结合蛋白促进 C6 大鼠神经胶质瘤细胞增殖并调节细胞周期
DOI：
10.3892/ijo.2017.4132
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发表时间：
2017-11
影响因子：
5.2
通讯作者：
Xia C
中科院分区：
文献类型：
--
作者：
Han X;Li H;Zhang Y;Qin J;Yang Q;Wang L;Yuan M;Xia C
神经胶质瘤是影响成人的最常见的原发性脑肿瘤。已经确定了四个等级的神经胶质瘤，即I-IV级。脑脂质结合蛋白（BLBP），其在脂肪酸的细胞内转运中起作用，在所有级别的人类胶质瘤中表达。将体外培养的胶质瘤细胞分为BLBP阳性和BLBP阴性细胞系。在本研究中，我们发现C6大鼠胶质瘤细胞是一种独特的BLBP阴性细胞系。我们的结果证实，在C6细胞中，外源BLBP的表达增加了增殖和S期细胞的百分比，在培养基中含有10或1%的FBS。此外，外源性BLBP在1%FBS培养基中下调肿瘤抑制因子p21和p16，而在10%FBS培养基中仅下调p21。异种移植模型试验的结果表明，外源性BLBP也刺激肿瘤形成和下调p21和p16。总之，我们的研究表明，外源性BLBP在体外促进C6细胞的增殖，并在体内促进肿瘤的形成。因此，BLBP在胶质瘤细胞中的表达可能通过抑制肿瘤抑制因子而促进细胞生长。
Gliomas are the most common primary brain tumors affecting adults. Four grades of gliomas have been identified, namely, grades I–IV. Brain lipid-binding protein (BLBP), which functions in the intracellular transport of fatty acids, is expressed in all grades of human gliomas. The glioma cells that are cultured in vitro are grouped into the BLBP-positive and BLBP-negative cell lines. In the present study, we found that C6 rat glioma cells was a distinct type of BLBP-negative cell line. Our results confirmed that in the C6 cells, the expression of exogenous BLBP increased the proliferation and percentage of cells in the S phase, in the culture medium containing 10 or 1% FBS. Moreover, exogenous BLBP was found to downregulate the tumor suppressors p21 and p16 in the 1% FBS culture medium, but only p21 in the 10% FBS culture medium. The results of the xenograft model assay showed that exogenous BLBP also stimulated tumor formation and downregulated p21 and p16. In conclusion, our study demonstrated that exogenous BLBP promoted proliferation of the C6 cells in vitro and facilitated tumor formation in vivo. Therefore, BLBP expression in glioma cells may promote cell growth by inhibiting the tumor suppressors.
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影响因子：
15.9
作者：
Dolecek, Therese A.;Propp, Jennifer M.;Kruchko, Carol
通讯作者：
Kruchko, Carol
DOI：
10.1016/j.bbrc.2011.06.152
发表时间：
2011-07-29
影响因子：
3.1
作者：
Hasan, Arif U.;Ohmori, Koji;Kohno, Masakazu
通讯作者：
Kohno, Masakazu
影响因子：
4
作者：
Krek, W
通讯作者：
Krek, W
影响因子：
5.6
作者：
Brun, Miranda;Coles, Jeffrey E.;Godbout, Roseline
通讯作者：
Godbout, Roseline
影响因子：
2.7
作者：
Duparc, Robert-Hugues;Abdouh, Mohamed;Bernier, Gilbert
通讯作者：
Bernier, Gilbert