Evidence for a digitalis-like substance in the hypothalamopituitary axis in rats: implications in the central cardiovascular regulation associated with an excess intake of sodium.
Evidence for a digitalis-like substance in the hypothalamopituitary axis in rats: implications in the central cardiovascular regulation associated with an excess intake of sodium.
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大鼠下丘脑垂体轴中存在洋地黄样物质的证据：与钠摄入过量相关的中枢心血管调节的影响。
DOI：
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Y. Ibata
中科院分区：
文献类型：
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作者：
Hakuo Takahashi;M. Matsuzawa;H. Okabayashi;K. Suga;I. Ikegaki;M. Yoshimura;Hamao Ijichi;Hitoshi Okamura;S. Murakami;Y. Ibata
本文用地高辛放射免疫分析法（RIA）测定了大鼠内源性洋地黄样物质的含量，并测定了其对哇巴因敏感的Na ~+，K ~+-ATP酶的抑制活性，探讨了内源性洋地黄样物质的来源及其生理作用。通过RIA使用从山羊中产生的抗体测量，洋地黄样物质在垂体中浓度高，而在下丘脑、肾上腺和其他器官中浓度降低。然而，肾上腺中洋地黄样物质的含量最高，这是通过从兔子体内产生的抗体测得的。用兔抗体测定，在2周的钠负荷期间，血浆水平显著降低，垂体切除时肾上腺含量显著降低。因此，用兔抗体测定的物质必须是ACTH依赖性肾上腺皮质类固醇之一。对Na+，K+-ATP酶的抑制活性在垂体中最高，肾上腺、下丘脑等器官依次降低。2周的钠负荷增加了脑下垂体的含量和尿液的排出量，并降低了下丘脑的含量。免疫组织化学染色显示，免疫反应仅限于室旁核、视上核和大细胞副核的神经元，其纤维延伸至正中隆起内层。为了确定该物质在脑中的作用，将溶解在人工脑脊液中的粗提取物注射到侧脑室中;记录持续超过30分钟的内脏神经的血管加压反应、心动过速和活动过度，其类似于对类似注射的哇巴因的反应。（250字处删节）
The origin and the physiological role of an endogenous digitalis-like substance were investigated by measuring both the digoxin-like substance by a digoxin radioimmunoassay (RIA) and the inhibitory activity on the ouabain sensitive Na+,K+-ATPase in rats. The digitalis-like substance was in high concentration in the pituitary, and in decreasing concentration in the hypothalamus, adrenal and the other organs as measured by RIA using an antibody raised from a goat. However, the adrenal showed the highest content of digitalis-like substance as measured by the antibody raised from a rabbit. The plasma level markedly decreased during a 2-week sodium-loading, and the adrenal content decreased markedly on hypophysectomy as measured with the rabbit-antibody. Therefore, the substance measured with the rabbit-antibody must be one of ACTH-dependent adrenal steroids. The inhibitory activity on the Na+,K+-ATPase was high in the pituitary gland, and was decreased in order of the adrenal, hypothalamus and other organs. The 2-week sodium-loading increased both the content in the pituitary gland and the output in the urine, and decreased the hypothalamic content. Immunohistochemical staining of the hypothalamus with the antibody revealed that the immunoreactivity is restricted to the neurons of the paraventricular nucleus, supraoptic nucleus, magnocellular accessory nuclei and extended their fibers reaching to the inner layer of the median eminence. To determine the role of the substance in the brain, the crude extract dissolved in artificial cerebrospinal fluid was injected into the lateral ventricle; vasopressor responses, tachycardia and hyperactivity of the splanchnic nerve lasting for more than 30 min were recorded, which resembled the responses to ouabain injected similarly.(ABSTRACT TRUNCATED AT 250 WORDS)
AV3V 病变对 DOCA 盐高血压和血管钠泵活性发展的影响。
DOI：
10.1161/01.hyp.4.5.575
发表时间：
1982
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
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作者：
Songu-Mize,E;Bealer,SL;Caldwell,RW
通讯作者：
Caldwell,RW
哇巴因对慢性良性扩张性高血压的小动脉对去甲肾上腺素反应的影响。
DOI：
10.1161/01.hyp.6.2_pt_2.i82
发表时间：
1984
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
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作者：
Overbeck,HW
通讯作者：
Overbeck,HW