Significance of anaerobes and oral bacteria in community-acquired pneumonia.
Significance of anaerobes and oral bacteria in community-acquired pneumonia.
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DOI：
10.1371/journal.pone.0063103
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Mukae H
中科院分区：
文献类型：
--
作者：
Yamasaki K;Kawanami T;Yatera K;Fukuda K;Noguchi S;Nagata S;Nishida C;Kido T;Ishimoto H;Taniguchi H;Mukae H
分子生物学方法具有较高的检出率，已被应用于鉴定引起传染病的细菌。大约10-48%的导致社区获得性肺炎的细菌病原体不能使用传统的培养方法进行鉴定。本研究使用支气管肺泡灌洗液标本16 S核糖体RNA基因的非培养型克隆文库分析评估社区获得性肺炎的细菌学原因，并将结果与常规培养方法的结果进行比较。社区获得性肺炎患者根据其临床和影像学表现入组。支气管肺泡灌洗标本收集从肺病理病变，使用支气管镜和评价两种培养的非依赖性分子方法和传统的培养方法。对于非培养物依赖的分子方法，使用聚合酶链反应用通用引物扩增约600个碱基对的16 S核糖体RNA基因，随后构建克隆文库。对每个样本随机选择的96个克隆的核苷酸序列进行测定，并搜索细菌同源性。常规培养方法，包括厌氧培养，也使用相同的标本进行。除了已知的社区获得性肺炎常见病原体[肺炎链球菌（18.8%）、流感嗜血杆菌（18.8%）、肺炎支原体（17.2%）]外，对64例社区获得性肺炎患者标本的分子分析显示，厌氧菌（15.6%）和口腔细菌（15.6%）的比例相对高于以往的报告。我们的研究结果表明，在社区获得性肺炎患者中检测到厌氧菌和口腔细菌的频率比之前认为的要高。这些细菌可能在社区获得性肺炎中发挥更重要的作用。
Molecular biological modalities with better detection rates have been applied to identify the bacteria causing infectious diseases. Approximately 10–48% of bacterial pathogens causing community-acquired pneumonia are not identified using conventional cultivation methods. This study evaluated the bacteriological causes of community-acquired pneumonia using a cultivation-independent clone library analysis of the 16S ribosomal RNA gene of bronchoalveolar lavage specimens, and compared the results with those of conventional cultivation methods. Patients with community-acquired pneumonia were enrolled based on their clinical and radiological findings. Bronchoalveolar lavage specimens were collected from pulmonary pathological lesions using bronchoscopy and evaluated by both a culture-independent molecular method and conventional cultivation methods. For the culture-independent molecular method, approximately 600 base pairs of 16S ribosomal RNA genes were amplified using polymerase chain reaction with universal primers, followed by the construction of clone libraries. The nucleotide sequences of 96 clones randomly chosen for each specimen were determined, and bacterial homology was searched. Conventional cultivation methods, including anaerobic cultures, were also performed using the same specimens. In addition to known common pathogens of community-acquired pneumonia [Streptococcus pneumoniae (18.8%), Haemophilus influenzae (18.8%), Mycoplasma pneumoniae (17.2%)], molecular analysis of specimens from 64 patients with community-acquired pneumonia showed relatively higher rates of anaerobes (15.6%) and oral bacteria (15.6%) than previous reports. Our findings suggest that anaerobes and oral bacteria are more frequently detected in patients with community-acquired pneumonia than previously believed. It is possible that these bacteria may play more important roles in community-acquired pneumonia.
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影响因子：
--
作者：
Marston, BJ;Plouffe, JF;Breiman, RF
通讯作者：
Breiman, RF
影响因子：
3.7
作者：
Erb-Downward JR;Thompson DL;Han MK;Freeman CM;McCloskey L;Schmidt LA;Young VB;Toews GB;Curtis JL;Sundaram B;Martinez FJ;Huffnagle GB
通讯作者：
Huffnagle GB
影响因子：
11.8
作者：
Johansson, Niclas;Kalin, Mats;Hedlund, Jonas
通讯作者：
Hedlund, Jonas
DOI：
10.1164/rccm.201111-2075oc
发表时间：
2012-05-15
影响因子：
24.7
作者：
Sze, Marc A.;Dimitriu, Pedro A.;Hogg, James C.
通讯作者：
Hogg, James C.
影响因子：
3
作者：
Miyashita, N;Fukano, H;Oka, M
通讯作者：
Oka, M