Analysis of the lung microbiome in the "healthy" smoker and in COPD.
Analysis of the lung microbiome in the "healthy" smoker and in COPD.
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DOI：
10.1371/journal.pone.0016384
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发表时间：
2011-02-22
期刊：
影响因子：
3.7
通讯作者：
Huffnagle GB
中科院分区：
文献类型：
--
作者：
Erb-Downward JR;Thompson DL;Han MK;Freeman CM;McCloskey L;Schmidt LA;Young VB;Toews GB;Curtis JL;Sundaram B;Martinez FJ;Huffnagle GB
尽管非培养技术已经表明肺不是无菌的，但对慢性阻塞性肺疾病(COPD)的肺微生物群知之甚少。我们使用16S扩增产物的焦磷酸测序以两种方式分析肺微生物群：第一，使用支气管肺泡灌洗(BAL)采样远端支气管和空气空间；第二，通过检查移植时切除的六名受试者肺中多个离散组织位置。我们对3名肺活量正常的不吸烟者(NS)、7名肺活量正常的吸烟者(“健康吸烟者”，HS)和4名COPD(CS)受试者进行了BAL。所有受试者均检出16条S序列，组间差异无统计学意义。基于分类学和分类学独立的方法都揭示了HS受试者之间细菌群落的异质性，这与在健康的NS和两名轻度COPD患者中看到的相似。中重度COPD患者的群落多样性非常有限，在28%的健康受试者中也存在这种情况。这两种方法都显示了三个研究小组的细菌群落之间存在广泛的成员重叠。没有一个属在一个组内是共同的，但在组间是独特的。我们的数据表明存在一个核心的肺部细菌微生物群，包括假单胞菌、链球菌、普雷沃特氏菌、梭杆菌、嗜血杆菌、韦氏杆菌和卟啉单胞菌。最引人注目的是，晚期COPD患者同一肺内的细菌群落有显著的显微解剖差异。这些研究进一步证明了肺部微生物群，并突出了严重COPD患者这些细菌群落的全局和微观解剖变化。
Although culture-independent techniques have shown that the lungs are not sterile, little is known about the lung microbiome in chronic obstructive pulmonary disease (COPD). We used pyrosequencing of 16S amplicons to analyze the lung microbiome in two ways: first, using bronchoalveolar lavage (BAL) to sample the distal bronchi and air-spaces; and second, by examining multiple discrete tissue sites in the lungs of six subjects removed at the time of transplantation. We performed BAL on three never-smokers (NS) with normal spirometry, seven smokers with normal spirometry (“heathy smokers”, HS), and four subjects with COPD (CS). Bacterial 16 s sequences were found in all subjects, without significant quantitative differences between groups. Both taxonomy-based and taxonomy-independent approaches disclosed heterogeneity in the bacterial communities between HS subjects that was similar to that seen in healthy NS and two mild COPD patients. The moderate and severe COPD patients had very limited community diversity, which was also noted in 28% of the healthy subjects. Both approaches revealed extensive membership overlap between the bacterial communities of the three study groups. No genera were common within a group but unique across groups. Our data suggests the existence of a core pulmonary bacterial microbiome that includes Pseudomonas, Streptococcus, Prevotella, Fusobacterium, Haemophilus, Veillonella, and Porphyromonas. Most strikingly, there were significant micro-anatomic differences in bacterial communities within the same lung of subjects with advanced COPD. These studies are further demonstration of the pulmonary microbiome and highlight global and micro-anatomic changes in these bacterial communities in severe COPD patients.
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高度平行的焦索测序者产生的16S rRNA序列的准确分类学分配。
影响因子：
14.9
作者：
Liu Z;DeSantis TZ;Andersen GL;Knight R
通讯作者：
Knight R
DOI：
10.1073/pnas.0308766101
发表时间：
2004-04-20
影响因子：
11.1
作者：
Lepp, PW;Brinig, MM;Relman, DA
通讯作者：
Relman, DA
DOI：
10.1073/pnas.0504978102
发表时间：
2005-08-02
影响因子：
11.1
作者：
Ley, RE;Bäckhed, F;Gordon, JI
通讯作者：
Gordon, JI
影响因子：
56.9
作者：
Murray, CJL;Lopez, AD
通讯作者：
Lopez, AD
影响因子：
6.4
作者：
Lemon, Katherine P.;Klepac-Ceraj, Vanja;Kolter, Roberto
通讯作者：
Kolter, Roberto