Reactive lysis: the complement-mediated lysis of unsensitized cells. I. The characterization of the indicator factor and its identification as C7.

Reactive lysis: the complement-mediated lysis of unsensitized cells. I. The characterization of the indicator factor and its identification as C7.
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反应性裂解:补体介导的未敏化细胞的裂解。 I.指标因子的表征及其标识为C7。

DOI:
10.1084/jem.131.4.629
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发表时间:
1970-04-01
影响因子:
15.3
通讯作者:
LACHMANN, PJ
LACHMANN, PJ
中科院分区:
医学1区
文献类型:
--
作者:
THOMPSON, RA;LACHMANN, PJ

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本文介绍了参与反应性溶血的指示因子(Ⅰ)的特性及其作为补体第七组分的鉴定。I被证明是一种β球蛋白,沉降系数为5.7S,分子量约为140,000。用抗I抗血清或活化的R优球蛋白消除I活性的实验表明,I是EAC 142阶段后细胞裂解所必需的迟效补体成分。纯化的I级分的补体成分分析排除了除C7之外的所有已知成分。I的理化特性与C7上已发表的数据一致。所述定量方法代表了C7的简便检测方法。
This paper describes the characteristics of the indicator factor (I) which takes part in reactive hemolysis and its identification as the seventh component of complement. I was shown to be a beta globulin with a sediment coefficient of 5.7S and a molecular weight of about 140,000. Experiments on the depletion of I activity with anti-I antiserum or with activated R euglobulin showed that I was a late acting complement component necessary for the lysis of cells after the EAC142 stage. Complement component analysis of purified I fractions excluded all known components except C7. The physicochemical characteristics of I are compatible with published data on C7. The method of quantitation described represents a convenient method of testing for C7.
DOI: 10.1084/jem.125.1.1
发表时间: 1967-01-01
期刊: The Journal of experimental medicine
影响因子: --
作者:
Nilsson UR;Müller-Eberhard HJ
通讯作者: Müller-Eberhard HJ
DOI: 10.1126/science.157.3787.442
发表时间: 1967-01-01
期刊: SCIENCE
影响因子: 56.9
作者:
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通讯作者: MULLEREB.HJ
DOI: 10.1016/0019-2791(66)90292-8
发表时间: 1966-01-01
期刊: IMMUNOCHEMISTRY
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DOI: 10.1084/jem.131.4.643
发表时间: 1970-01-01
影响因子: 15.3
作者:
LACHMANN, PJ;THOMPSON, RA
通讯作者: THOMPSON, RA
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