Functional Characterization of CsSWEET5a, a Cucumber Hexose Transporter That Mediates the Hexose Supply for Pollen Development and Rescues Male Fertility in Arabidopsis.
Functional Characterization of CsSWEET5a, a Cucumber Hexose Transporter That Mediates the Hexose Supply for Pollen Development and Rescues Male Fertility in Arabidopsis.
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DOI：
10.3390/ijms25021332
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发表时间：
2024-01-22
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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花粉细胞需要花药中的大量糖来支持其发育，这对植物有性生殖和作物产量至关重要。糖最终将被输出转运蛋白（Sugars Will Finally Be Exported Transporters，SWEERs）是将花药组织中的糖从质外体卸载到共质体分离的发育中的花粉细胞中的重要转运蛋白，从而影响花粉发育过程中糖的供应。然而，在黄瓜（Cucumis sativus L.）在基因组中，参与这一过程的CsSWEET基因尚未确定。在这里，SWEET基因家族的成员，CsSWEET 5a，被确定和表征。实时荧光定量PCR和β-葡萄糖醛酸酶表达分析表明，CsSWEET 5a在黄瓜雄花的花药和花粉细胞中从小孢子母细胞期（第9期）到成熟花粉期（第12期）都有高表达。其亚细胞定位表明CsSWEET 5a蛋白定位于质膜。在酵母中的异源表达试验表明，CsSWEET 5a编码的己糖转运蛋白，可以补充葡萄糖和果糖转运缺陷。CsSWEET 5a可以显著拯救atsweet 8突变体拟南芥植物的花粉生活力和育性。CsSWEET 5a可能通过质外体向发育中的花粉细胞供应己糖。
Pollen cells require large amounts of sugars from the anther to support their development, which is critical for plant sexual reproduction and crop yield. Sugars Will Eventually be Exported Transporters (SWEETs) have been shown to play an important role in the apoplasmic unloading of sugars from anther tissues into symplasmically isolated developing pollen cells and thereby affect the sugar supply for pollen development. However, among the 17 CsSWEET genes identified in the cucumber (Cucumis sativus L.) genome, the CsSWEET gene involved in this process has not been identified. Here, a member of the SWEET gene family, CsSWEET5a, was identified and characterized. The quantitative real-time PCR and β-glucuronidase expression analysis revealed that CsSWEET5a is highly expressed in the anthers and pollen cells of male cucumber flowers from the microsporocyte stage (stage 9) to the mature pollen stage (stage 12). Its subcellular localization indicated that the CsSWEET5a protein is localized to the plasma membrane. The heterologous expression assays in yeast demonstrated that CsSWEET5a encodes a hexose transporter that can complement both glucose and fructose transport deficiencies. CsSWEET5a can significantly rescue the pollen viability and fertility of atsweet8 mutant Arabidopsis plants. The possible role of CsSWEET5a in supplying hexose to developing pollen cells via the apoplast is also discussed.
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