Unraveling the roles of CD44/CD24 and ALDH1 as cancer stem cell markers in tumorigenesis and metastasis.
Unraveling the roles of CD44/CD24 and ALDH1 as cancer stem cell markers in tumorigenesis and metastasis.
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DOI：
10.1038/s41598-017-14364-2
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发表时间：
2017-10-23
影响因子：
4.6
通讯作者：
Yang Y
中科院分区：
文献类型：
--
作者：
Li W;Ma H;Zhang J;Zhu L;Wang C;Yang Y
CD 44/CD 24和ALDH 1是乳腺癌中广泛使用的癌症干细胞（CSC）标志物。然而，它们的表达并不总是一致的，即使在相同亚型的乳腺癌中。对它们的功能还缺乏系统的比较。我们研究了CD 44、CD 24和ALDH 1在不同亚型乳腺癌细胞中的表达，并探讨了它们与乳腺癌进展的关系。我们定义了一个参数CD 44/CD 24比值来表示CD 44和CD 24的表达水平，发现高CD 44/CD 24比值和ALDH 1+都是恶性肿瘤的指标，但在肿瘤进展过程中发挥不同的作用。高的CD 44/CD 24比值与细胞增殖和肿瘤发生更相关，这一点在异种移植小鼠的乳腺球形成和肿瘤发生中得到证实。ALDH 1+是细胞迁移和肿瘤转移的更强指标。通过siRNA抑制CD 44和ALDH 1导致致瘤性和细胞迁移能力降低。高CD 44/CD 24比值和ALDH 1+的组合将是表征CSC的更可靠的方法。此外，高CD 44/CD 24比值和ALDH 1+在从原发肿瘤到循环肿瘤细胞（CTC）和远处转移的转移过程中是保守的，这表明这些CSC标志物在辅助癌症检测、预后评估甚至癌症治疗方面具有重要价值。
CD44/CD24 and ALDH1 are widely used cancer stem cell (CSC) markers in breast cancer. However, their expression is not always consistent even in the same subtype of breast cancer. Systematic comparison of their functions is still lacking. We investigated the expression of CD44, CD24 and ALDH1 in different subtypes of breast cancer cells, and explored their relationship with cancer progression. We defined a parameter CD44/CD24 ratio to present the expression level of CD44 and CD24 and found that high CD44/CD24 ratio and ALDH1+ are both indicators for cancer malignancy, but play different functions during tumor progression. High CD44/CD24 ratio is more related to cell proliferation and tumorigenesis, which is confirmed by mammosphere formation and tumorigenesis in xenotransplanted mice. ALDH1+ is a stronger indicator for cell migration and tumor metastasis. Suppression of CD44 and ALDH1 by siRNA led to decreased tumorigenicity and cell migration capacity. The combination of high CD44/CD24 ratio and ALDH1+ would be a more reliable way to characterize CSCs. Moreover, both high CD44/CD24 ratio and ALDH1+ were conserved during metastasis, from the primary tumors to the circulating tumor cells (CTCs) and the distant metastases, suggesting the significant value of these CSC markers in assisting cancer detection, prognostic evaluation, and even cancer therapeutics.
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影响因子：
10.5
作者：
Dontu, G;Abdallah, WM;Wicha, MS
通讯作者：
Wicha, MS
DOI：
10.1073/pnas.0603806103
发表时间：
2006-08-01
影响因子：
11.1
作者：
Chute, John P.;Muramoto, Garrett G.;McDonnell, Donald P.
通讯作者：
McDonnell, Donald P.
影响因子：
5.9
作者：
Liu S;Cong Y;Wang D;Sun Y;Deng L;Liu Y;Martin-Trevino R;Shang L;McDermott SP;Landis MD;Hong S;Adams A;D'Angelo R;Ginestier C;Charafe-Jauffret E;Clouthier SG;Birnbaum D;Wong ST;Zhan M;Chang JC;Wicha MS
通讯作者：
Wicha MS
了解和靶向癌症干细胞：治疗意义和挑战
影响因子：
8.2
作者：
Chen, Ke;Huang, Ying-hui;Chen, Ji-long
通讯作者：
Chen, Ji-long
影响因子：
2.8
作者：
Dong, Yi;Bi, Li-Rong;Fan, Zhi-Min
通讯作者：
Fan, Zhi-Min