Multiple-Streams Focusing-Based Cell Separation in High Viscoelasticity Flow.
Multiple-Streams Focusing-Based Cell Separation in High Viscoelasticity Flow.
复制标题
DOI：
10.1021/acsomega.2c06021
复制
发表时间：
2022-11-15
期刊：
影响因子：
4.1
通讯作者：
Gale, Bruce K.
中科院分区：
文献类型：
--
作者：
Feng, Haidong;Patel, Dhruv;Magda, Jules J.;Geher, Sage;Sigala, Paul A.;Gale, Bruce K.
粘弹性流动广泛应用于微流控颗粒分离过程中，其中颗粒在稀释的粘弹性流动中集中在通道中心。在本文中，从单流聚焦过渡到多流聚焦（MSF）在高粘弹性流动观察，这是适用于细胞分离过程。颗粒聚焦流分叉是由弹性力和粘弹性二次流阻力的平衡引起的。详细研究了细胞的物理性质，如细胞的尺寸，形状和变形能力，对多流聚焦的形成的影响。利用不同的分离标准实现颗粒分离。证明了红（RBC）和白色（WBC）血细胞的基于尺寸的分离，其中细胞基于它们的尺寸差异被聚焦在不同的流中。具有不同变形能力的细胞在粘弹性流动中被拉伸，导致聚焦流的变化，这种特性被用来分离感染疟疾寄生虫恶性疟原虫的红细胞。所取得的成果促进了我们对高粘弹性流动中颗粒运动的理解，并使新的颗粒操纵和分离过程的样品处理在生物流体。
Viscoelastic flow has been widely used in microfluidic particle separation processes, in which particles get focused on the channel center in diluted viscoelastic flow. In this paper, the transition from single-stream focusing to multiple-streams focusing (MSF) in high viscoelastic flow is observed, which is applied for cell separation processes. Particle focusing stream bifurcation is caused by the balance between elastic force and viscoelastic secondary flow drag force. The influence of cell physical properties, such as cell dimension, shape, and deformability, on the formation of multiple-streams focusing is studied in detail. Particle separation is realized utilizing different separation criteria. The size-based separation of red (RBC) and white (WBC) blood cells is demonstrated in which cells get focused in different streams based on their dimension difference. Cells with different deformabilities get stretched in the viscoelastic flow, leading to the change of focusing streams, and this property is harnessed to separate red blood cells infected with the malaria parasite, Plasmodium falciparum. The achieved results promote our understanding of particle movement in the high viscoelastic flow and enable new particle manipulation and separation processes for sample treatment in biofluids.
登录
查看更多内容
影响因子：
3.4
作者：
Feng H;Jafek AR;Wang B;Brady H;Magda JJ;Gale BK
通讯作者：
Gale BK
影响因子：
6.1
作者：
D'Avino, Gaetano;Romeo, Giovanni;Maffettone, Pier Luca
通讯作者：
Maffettone, Pier Luca
影响因子：
5.7
作者：
Frank SM;Abazyan B;Ono M;Hogue CW;Cohen DB;Berkowitz DE;Ness PM;Barodka VM
通讯作者：
Barodka VM
DOI：
10.1073/pnas.0704958104
发表时间：
2007-11-27
影响因子：
11.1
作者：
Di Carlo, Dino;Irimia, Daniel;Toner, Mehmet
通讯作者：
Toner, Mehmet
影响因子：
4.6
作者：
Jafek A;Feng H;Brady H;Petersen K;Chaharlang M;Aston K;Gale B;Jenkins T;Samuel R
通讯作者：
Samuel R