An automated instrument for intrauterine insemination sperm preparation.
An automated instrument for intrauterine insemination sperm preparation.
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DOI：
10.1038/s41598-020-78390-3
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发表时间：
2020-12-07
影响因子：
4.6
通讯作者：
Samuel R
中科院分区：
文献类型：
--
作者：
Jafek A;Feng H;Brady H;Petersen K;Chaharlang M;Aston K;Gale B;Jenkins T;Samuel R
精子准备是成功实现宫腔内授精的关键，需要处理精液样本以去除白色血细胞，洗去精浆，并减少样本体积。我们提出了一个自动化的仪器能够进行精子制备与稀释精液样本开始。我们通过在每次治疗中处理0.5 mL的患者样本，将我们的设备与密度梯度离心进行比较。在5分钟的操作时间内，该仪器从原始样本中平均回收86%的所有精子和82%的渐进活动精子，同时去除白色血细胞，替换精浆，并将样本体积减少到临床所需的水平。在25分钟的操作时间内，密度梯度离心平均回收33%的精子和41%的渐进运动精子。自动化仪器可以通过允许卫星医生办公室为没有现有方法所需的临床支持的患者提供子宫内授精作为一种选择来改善IUI作为一种治疗选择的可及性。
Sperm preparation is critical to achieving a successful intrauterine insemination and requires the processing of a semen sample to remove white blood cells, wash away seminal plasma, and reduce sample volume. We present an automated instrument capable of performing a sperm preparation starting with a diluted semen sample. We compare our device against a density gradient centrifugation by processing 0.5 mL portions of patient samples through each treatment. In 5 min of operating time, the instrument recovers an average of 86% of all sperm and 82% of progressively motile sperm from the original sample while removing white blood cells, replacing the seminal plasma, and reducing the volume of the sample to the clinically required level. In 25 min of operating time, density gradient centrifugation recovers an average of 33% of all sperm and 41% of progressively motile sperm. The automated instrument could improve access to IUI as a treatment option by allowing satellite doctor’s offices to offer intrauterine insemination as an option for patients without the clinical support required by existing methods.
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影响因子：
1.6
作者：
Muratori M;Tarozzi N;Cambi M;Boni L;Iorio AL;Passaro C;Luppino B;Nadalini M;Marchiani S;Tamburrino L;Forti G;Maggi M;Baldi E;Borini A
通讯作者：
Borini A
影响因子：
16.6
作者：
Lim, Eugene J.;Ober, Thomas J.;Edd, Jon F.;Desai, Salil P.;Neal, Douglas;Bong, Ki Wan;Doyle, Patrick S.;McKinley, Gareth H.;Toner, Mehmet
通讯作者：
Toner, Mehmet
影响因子：
6.1
作者：
Kuntaegowdanahalli, Sathyakumar S.;Bhagat, Ali Asgar S.;Papautsky, Ian
通讯作者：
Papautsky, Ian
影响因子：
6.1
作者：
Nosrati, Reza;Vollmer, Marion;Sinton, David
通讯作者：
Sinton, David
影响因子：
13.3
作者：
Cooper, Trevor G.;Noonan, Elizabeth;Vogelsong, Kirsten M.
通讯作者：
Vogelsong, Kirsten M.