Stereoselective Production of Dimethyl-Substituted Carbapenams via Engineered Carbapenem Biosynthesis Enzymes
Stereoselective Production of Dimethyl-Substituted Carbapenams via Engineered Carbapenem Biosynthesis Enzymes
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通过工程碳青霉烯生物合成酶立体选择性生产二甲基取代的碳青霉烯类
DOI：
10.1021/acscatal.6b02509
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发表时间：
2017
期刊：
影响因子：
12.9
通讯作者：
Hamed R
中科院分区：
文献类型：
--
作者：
Hamed R
Stereoselective biocatalysis by crotonase superfamily enzymes is exemplified by use of engineered 5-carboxymethylproline synthases (CMPSs) for preparation of functionalized 5-carboxymethylproline (5-CMP) derivatives methylated at two positions (i.e., C2/C6, C3/C6, and C5/C6), including products with a quaternary center, from appropriately substituted-amino acid aldehydes and C-2 epimeric methylmalonyl-CoA. The enzymatically produced disubstituted 5-CMPs were converted by carbapenam synthetase into methylated bicyclic β-lactams, which manifestly improved hydrolytic stability compared to the unsubstituted carbapenams. The results highlight the use of modified carbapenem biosynthesis enzymes for production of carbapenams with improved properties.
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DOI：
10.1021/jo4017953
发表时间：
2013-12-20
期刊：
The Journal of organic chemistry
影响因子：
--
作者：
Repka LM;Reisman SE
通讯作者：
Reisman SE
通过工程化巴豆酸酶与烷基丙二酸单酰辅酶A合成酶的偶联，立体选择性地制备脂质化羧甲基脯氨酸/哌可酸衍生物。
影响因子：
3.2
作者：
Hamed RB
通讯作者：
Hamed RB
青霉素的生物合成；
影响因子：
4.8
作者：
O. Behrens;J. Corse
通讯作者：
J. Corse
影响因子：
3.6
作者：
McGowan, SJ;Sebaihia, M;Salmond, GPC
通讯作者：
Salmond, GPC
工程化羧甲基脯氨酸合酶催化立体选择性 C-C 键形成。
影响因子：
21.8
作者：
Hamed RB
通讯作者：
Hamed RB