Dual-energy micro-CT functional imaging of primary lung cancer in mice using gold and iodine nanoparticle contrast agents: a validation study.
Dual-energy micro-CT functional imaging of primary lung cancer in mice using gold and iodine nanoparticle contrast agents: a validation study.
复制标题
DOI：
10.1371/journal.pone.0088129
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Badea CT
中科院分区：
文献类型：
--
作者：
Ashton JR;Clark DP;Moding EJ;Ghaghada K;Kirsch DG;West JL;Badea CT
为了给肿瘤定性提供更多的功能信息，我们研究了双能量计算机断层成像技术在小鼠肺肿瘤成像中的应用。使用金和碘纳米颗粒对肿瘤血容量和血管通透性进行定量。并将该方法与单一造影剂/单能量CT方法进行比较。进行了体外验证研究，以证明CT在体内定量造影剂的准确性。LSL-KrasG12D；p53FL/FL小鼠产生原发肺癌。注射金纳米粒子，两天后注射碘纳米粒子。金在肿瘤中积累，而碘提供血管内对比剂。进行了三次双能量CT扫描--两次为单造影剂扫描，一次为双造影剂扫描。每一次扫描中的金和碘浓度都是使用双能量分解法计算的。对于每种方法，根据碘浓度计算肿瘤的血容量分数，并根据累积的金浓度估计肿瘤血管的通透性。为了验证，将CT测量结果与组织学和电感耦合等离子体光发射光谱测量组织中的金浓度进行了比较。双能量CT能够在体内分离金和碘造影剂，并显示金纳米颗粒在脾、肝和肿瘤中的摄取。两种成像方法测得的肿瘤血容量分数一致，且与组织学微血管密度有较高的相关性(R2 = 0.81)。两种成像方法获得的血管通透性测量结果与体外测量结果吻合较好。使用两种纳米颗粒的双能量CT相当于单纳米颗粒方法，但允许通过一次扫描测量血液体积分数和通透性。正如体外方法证实的那样，CT衍生的纳米颗粒浓度是准确的。该方法对肺部肿瘤的CT定性诊断具有重要意义。
To provide additional functional information for tumor characterization, we investigated the use of dual-energy computed tomography for imaging murine lung tumors. Tumor blood volume and vascular permeability were quantified using gold and iodine nanoparticles. This approach was compared with a single contrast agent/single-energy CT method. Ex vivo validation studies were performed to demonstrate the accuracy of in vivo contrast agent quantification by CT. Primary lung tumors were generated in LSL-KrasG12D; p53FL/FL mice. Gold nanoparticles were injected, followed by iodine nanoparticles two days later. The gold accumulated in tumors, while the iodine provided intravascular contrast. Three dual-energy CT scans were performed–two for the single contrast agent method and one for the dual contrast agent method. Gold and iodine concentrations in each scan were calculated using a dual-energy decomposition. For each method, the tumor fractional blood volume was calculated based on iodine concentration, and tumor vascular permeability was estimated based on accumulated gold concentration. For validation, the CT-derived measurements were compared with histology and inductively-coupled plasma optical emission spectroscopy measurements of gold concentrations in tissues. Dual-energy CT enabled in vivo separation of gold and iodine contrast agents and showed uptake of gold nanoparticles in the spleen, liver, and tumors. The tumor fractional blood volume measurements determined from the two imaging methods were in agreement, and a high correlation (R2 = 0.81) was found between measured fractional blood volume and histology-derived microvascular density. Vascular permeability measurements obtained from the two imaging methods agreed well with ex vivo measurements. Dual-energy CT using two types of nanoparticles is equivalent to the single nanoparticle method, but allows for measurement of fractional blood volume and permeability with a single scan. As confirmed by ex vivo methods, CT-derived nanoparticle concentrations are accurate. This method could play an important role in lung tumor characterization by CT.
登录
查看更多内容
影响因子：
3.7
作者：
Karathanasis E;Chan L;Karumbaiah L;McNeeley K;D'Orsi CJ;Annapragada AV;Sechopoulos I;Bellamkonda RV
通讯作者：
Bellamkonda RV
影响因子：
7.4
作者：
Haiss, Wolfgang;Thanh, Nguyen T. K.;Fernig, David G.
通讯作者：
Fernig, David G.
DOI：
10.1093/jnci/djn389
发表时间：
2008-12-03
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
Jemal A;Thun MJ;Ries LA;Howe HL;Weir HK;Center MM;Ward E;Wu XC;Eheman C;Anderson R;Ajani UA;Kohler B;Edwards BK
通讯作者：
Edwards BK
双能和多能 CT：功能成像方法。
影响因子：
4.7
作者：
Fornaro, Juergen;Leschka, Sebastian;Hibbeln, Dennis;Butler, Anthony;Anderson, Nigel;Pache, Gregor;Scheffel, Hans;Wildermuth, Simon;Alkadhi, Hatem;Stolzmann, Paul
通讯作者：
Stolzmann, Paul
影响因子：
1.9
作者：
Hodnett, Philip A.;Ko, Jane P.
通讯作者：
Ko, Jane P.