The mitochondrial genome sequence and molecular phylogeny of the turkey, Meleagris gallopavo.

The mitochondrial genome sequence and molecular phylogeny of the turkey, Meleagris gallopavo.
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DOI:
10.1111/j.1365-2052.2008.01810.x
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发表时间:
2009-04
期刊:
影响因子:
2.4
通讯作者:
Smith EJ
Smith EJ
中科院分区:
生物学3区
文献类型:
--
作者:
Guan X;Silva P;Gyenai KB;Xu J;Geng T;Tu Z;Samuels DC;Smith EJ

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火鸡(Meleagris gallopavo)的线粒体基因组(mtGenome)研究很少,没有公开的全基因组线粒体序列。在这里,我们使用基于 PCR 的方法,使用从鸡和其他物种设计的 19 对引物来开发完整的火鸡 mtGenome 序列。获得了全长16, 717 bp的火鸡mtGenome,与鸡mtGenome相似度为85%。注释了 13 个基因和 24 个 RNA(22 个 tRNA 和 2 个 rRNA)。基于线粒体基因组的系统发育分析表明,火鸡与鸡(Gallus gallus)和鹌鹑(Corturnix japonica)关系最为密切。鉴于线粒体基因组的重要性,目前的工作增加了定义火鸡某些经济性状背后的遗传机制所需的不断增长的基因组资源。
The mitochondrial genome (mtGenome) has been very little studied in the turkey (Meleagris gallopavo), for which there is no publicly available whole genome mitochondrial sequence. Here, we used PCR-based methods with 19 pairs of primers designed from the chicken and other species to develop a complete turkey mtGenome sequence. A total length of 16, 717 bp of the whole turkey mtGenome was obtained, with 85% similarity to chicken mtGenome. There were 13 genes and 24 RNA (22 tRNA and 2 rRNA) annotated. The mtGenome-based phylogenetic analysis suggests that the turkey is most closely related to the chicken, Gallus gallus, and quail, Corturnix japonica. Given the importance of the mitochondria genome, the present work adds to the growing genomic resources needed to define the genetic mechanisms that underlie some economic traits in the turkey.
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