STV11 encodes a sulphotransferase and confers durable resistance to rice stripe virus.
STV11 encodes a sulphotransferase and confers durable resistance to rice stripe virus.
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DOI：
10.1038/ncomms5768
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发表时间：
2014-09-09
影响因子：
16.6
通讯作者：
Wan, Jianmin
中科院分区：
文献类型：
--
作者：
Wang, Qi;Liu, Yuqiang;He, Jun;Zheng, Xiaoming;Hu, Jinlong;Liu, Yanling;Dai, Huimin;Zhang, Yingxin;Wang, Baoxiang;Wu, Weixun;Gao, He;Zhang, Yunhui;Tao, Xiaorong;Deng, Huafeng;Yuan, Dingyang;Jiang, Ling;Zhang, Xin;Guo, Xiuping;Cheng, Xianian;Wu, Chuanyin;Wang, Haiyang;Yuan, Longping;Wan, Jianmin
水稻条纹病毒（Rice stripe virus，RSV）是水稻上最严重的病毒病之一，但RSV抗性的分子基础仍然难以捉摸。在这里，我们表明，水稻STV 11（STV 11-R）的抗性等位基因编码的磺基转移酶（OsSOT 1）催化水杨酸（SA）转化为磺化SA（SSA），而敏感等位基因STV 11-S编码的基因产物失去了这种活性。序列分析表明，STV 11-R和STV 11-S等位基因在不同地理群体的野生稻，Oryza rufipogon的预分化，并保持普遍的栽培籼稻和粳稻品种，分别。将STV 11-R等位基因渗入到感病品种中或将STV 11-R异源转移到烟草植物中赋予对RSV的有效抗性。我们的研究结果为植物病毒防御机制提供了新的见解，并提出了利用分子标记辅助选择或基因工程培育抗RSV作物的有效方法。 水稻条纹病毒（RSV）引起水稻病害，具有显著的经济后果。在这里，作者在水稻中克隆了一个抗RSV基因，并提出该基因编码一种磺基转移酶，催化水杨酸（SA）转化为磺化水杨酸，导致RSV感染植物中SA积累增加并抑制病毒复制。
Rice stripe virus (RSV) causes one of the most serious viral diseases of rice (Oryza sativa L.), but the molecular basis of RSV resistance has remained elusive. Here we show that the resistant allele of rice STV11 (STV11-R) encodes a sulfotransferase (OsSOT1) catalysing the conversion of salicylic acid (SA) into sulphonated SA (SSA), whereas the gene product encoded by the susceptible allele STV11-S loses this activity. Sequence analyses suggest that the STV11-R and STV11-S alleles were predifferentiated in different geographic populations of wild rice, Oryza rufipogon, and remained prevalent in cultivated indica and japonica rice varieties, respectively. Introgression of the STV11-R allele into susceptible cultivars or heterologous transfer of STV11-R into tobacco plants confers effective resistance against RSV. Our results shed new insights into plant viral defense mechanisms and suggest effective means of breeding RSV-resistant crops using molecular marker-assisted selection or genetic engineering. Rice stripe virus (RSV) causes a disease in rice with significant economic consequences. Here, the authors clone an RSV-resistant gene in rice and suggest that this gene encodes a sulphotransferase that catalyses the conversion of salicylic acid (SA) into sulphonated salicylic acid, leading to increased SA accumulation in RSV-infected plants and inhibition of viral replication.
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影响因子：
56.9
作者：
DELANEY, TP;UKNES, S;RYALS, J
通讯作者：
RYALS, J
影响因子：
5.1
作者：
Huang, WE;Wang, H;Whiteley, AS
通讯作者：
Whiteley, AS
影响因子：
5.2
作者：
Otuka A
通讯作者：
Otuka A
影响因子：
5.4
作者：
Hayano-Saito, Y;Saito, K;Iwasaki, M
通讯作者：
Iwasaki, M
影响因子：
7.2
作者：
Boevink, P;Oparka, K;Hawes, C
通讯作者：
Hawes, C